European Synchrotron Radiation Facility, Grenoble, France.
Department of Biological Science and Program in Neuroscience, Florida State University, Tallahassee, FL, USA.
Sci Rep. 2017 Oct 30;7(1):14290. doi: 10.1038/s41598-017-14757-3.
Microbeam radiation therapy is a novel preclinical technique, which uses synchrotron-generated X-rays for the treatment of brain tumours and drug-resistant epilepsies. In order to safely translate this approach to humans, a more in-depth knowledge of the long-term radiobiology of microbeams in healthy tissues is required. We report here the result of the characterization of the rat sensorimotor cortex tolerance to microradiosurgical parallel transections. Healthy adult male Wistar rats underwent irradiation with arrays of parallel microbeams. Beam thickness, spacing and incident dose were 100 or 600 µm, 400 or 1200 µm and 360 or 150 Gy, respectively. Motor performance was carried over a 3-month period. Three months after irradiation rats were sacrificed to evaluate the effects of irradiation on brain tissues by histology and immunohistochemistry. Microbeam irradiation of sensorimotor cortex did not affect weight gain and motor performance. No gross signs of paralysis or paresis were also observed. The cortical architecture was not altered, despite the presence of cell death along the irradiation path. Reactive gliosis was evident in the microbeam path of rats irradiated with 150 Gy, whereas no increase was observed in rats irradiated with 360 Gy.
微束放射治疗是一种新颖的临床前技术，它使用同步加速器产生的 X 射线来治疗脑肿瘤和耐药性癫痫。为了安全地将这种方法转化为人类，我们需要更深入地了解微束在健康组织中的长期放射生物学。我们在这里报告了对大鼠感觉运动皮层对微放射手术平行横断耐受性进行特征描述的结果。健康成年雄性 Wistar 大鼠接受平行微束阵列照射。光束厚度、间距和入射剂量分别为 100 或 600μm、400 或 1200μm 和 360 或 150Gy。在 3 个月的时间内进行运动性能评估。照射后 3 个月，处死大鼠，通过组织学和免疫组织化学评估照射对脑组织的影响。感觉运动皮层的微束照射不会影响体重增加和运动性能。也没有观察到明显的瘫痪或轻瘫迹象。尽管在照射路径沿线存在细胞死亡，但皮质结构没有改变。在接受 150Gy 照射的大鼠中，微束路径中存在明显的反应性神经胶质增生，而在接受 360Gy 照射的大鼠中没有观察到这种增生。
