Su Ao-Lei, Lan Xiu-Wan, Huang Ming-Bo, Nong Wei, Li Qingdi Quentin, Leng Jing
Department of Microbiology and Immunology, Key Laboratory for Complementary and Alternative Medicine Experimental Animal Models of Guangxi, Guangxi University of Chinese Medicine, Nanning, Guangxi 530001, P.R. China.
Department of Basic Medical Training Center, Guangxi Medical College, Nanning, Guangxi 530021, P.R. China.
Biomed Rep. 2017 Oct;7(4):349-352. doi: 10.3892/br.2017.969. Epub 2017 Aug 18.
The Chinese tree shrew () has long been proposed to serve as an animal model for studying human diseases. However, its overall genetic diversity and population structure remain largely unknown. In the present study, we investigated the genetic diversity of population microsatellite DNA in wild . Sixteen microsatellite loci were assessed in 76 wild . The target microsatellite DNA fragments were amplified from the peripheral blood DNA of the animals by polymerase chain reaction (PCR), and the PCR-amplified products were verified by DNA sequencing and used for the analysis of allele, effective allele, genetic heterozygosity, polymorphism and population structure. Our results showed that of the 16 microsatellite loci examined, 5 microsatellite loci were monomorphic and 11 microsatellite loci were polymorphic. We detected 61 alleles in the polymorphic loci and found 2-10 (with an average of 5.5455) alleles per locus. Our data also showed that the observed and expected heterozygosities ranged from 0.087 to 0.8947 and 0.1368 to 0.7892 with an average of 0.3968 and 0.4796, respectively. Taken together, the results revealed a considerably high heterozygosity and high genetic diversity at the molecular level in the population of wild . The identified markers from the present study may be useful for individual identification and parentage testing, as well as for the quantification of population heterogeneity in the Chinese tree shrew.
中国树鼩长期以来一直被提议作为研究人类疾病的动物模型。然而，其整体遗传多样性和种群结构在很大程度上仍然未知。在本研究中，我们调查了野生中国树鼩种群微卫星DNA的遗传多样性。在76只野生中国树鼩中评估了16个微卫星位点。通过聚合酶链反应（PCR）从动物外周血DNA中扩增目标微卫星DNA片段，并通过DNA测序对PCR扩增产物进行验证，用于等位基因、有效等位基因、遗传杂合度、多态性和种群结构分析。我们的结果表明，在所检测的16个微卫星位点中，5个微卫星位点是单态的，11个微卫星位点是多态的。我们在多态位点检测到61个等位基因，每个位点发现2至10个（平均为5.5455个）等位基因。我们的数据还表明，观察到的和预期的杂合度范围分别为0.087至0.8947和0.1368至0.7892，平均分别为0.3968和0.4796。综上所述，结果揭示了野生中国树鼩种群在分子水平上具有相当高的杂合度和高遗传多样性。本研究中鉴定的标记可能有助于个体识别和亲子鉴定，以及量化中国树鼩种群的异质性。
