Bai C-C, Liu S-F, Zhuang Z-M, Lin L, Wang H, Chang Y-Q
Key Laboratory of Mariculture, Ministry of Agriculture, Dalian Ocean University, Dalian, China.
Genet Mol Res. 2011 Sep 15;10(3):2065-8. doi: 10.4238/vol10-3gmr1522.
The Korean rockfish (Sebastes schlegeli) is an important commercial fish that is widely used in aquaculture. We isolated and characterized 18 polymorphic microsatellite loci from the Korean rockfish using a (GT)(13)-enriched genomic library. Polymorphism was assessed in 48 individuals from a single population collected from the northern coastal waters of the Yellow Sea. The observed and expected heterozygosities ranged from 0.0244 to 0.7660 (mean 0.4194) and 0.0244 to 0.8758 (mean 0.5002), respectively. Polymorphism at these loci indicated from two to 15 alleles (mean 5.7); 14 of 18 loci conformed to Hardy-Weinberg equilibrium. These markers should be useful for management and conservation studies of this species.
许氏平鲉是一种重要的商业鱼类，广泛应用于水产养殖。我们利用富含(GT)(13)的基因组文库从许氏平鲉中分离并鉴定了18个多态微卫星位点。对从黄海北部沿海水域采集的单一群体中的48个个体进行了多态性评估。观察到的杂合度和预期杂合度分别为0.0244至0.7660（平均0.4194）和0.0244至0.8758（平均0.5002）。这些位点的多态性显示有2至15个等位基因（平均5.7个）；18个位点中的14个符合哈迪-温伯格平衡。这些标记对于该物种的管理和保护研究应是有用的。
