Gross A, Weiss E, Tapia F J, Aranzazu N, Gallinoto M E, Convit J
Instituto de Biomedicina, Caracas, Venezuela.
Am J Trop Med Hyg. 1988 May;38(3):608-12. doi: 10.4269/ajtmh.1988.38.608.
Leukocyte subsets present in the granulomatous response produced after the inoculation of a mixture of Mycobacterium leprae and BCG in lepromatous leprosy patients were characterized in situ using monoclonal antibodies and an immunoperoxidase technique. The granuloma produced after M. leprae-BCG inoculation showed a distribution pattern similar to tuberculoid granulomas. T lymphocytes bearing the CD8 phenotype (T cytotoxic/suppressor) were sequestered to the periphery of the epithelioid tubercles and T helper-inducer CD4+ lymphocytes were distributed throughout the infiltrate. Langerhans cells CD1+ were increased in the epidermis, and in dermis they were localized mainly in the mantle surrounding the granuloma. Most of the dermal infiltrate produced after the inoculation or M. leprae-BCG expresses the HLA-DR antigen. Similarly, most keratinocytes were also positive to this MHC antigen. The granulomatous response to BCG was similar to the inoculation of a mixture of M. leprae-BCG, however acid-fast bacilla were still present. The inoculation of M. leprae produced a macrophage granuloma with no clearing of the bacilla which resembles the lepromatous leprosy granuloma.
利用单克隆抗体和免疫过氧化物酶技术，对瘤型麻风患者接种麻风分枝杆菌和卡介苗混合物后产生的肉芽肿反应中的白细胞亚群进行原位特征分析。接种麻风分枝杆菌 - 卡介苗后产生的肉芽肿显示出与结核样肉芽肿相似的分布模式。带有CD8表型的T淋巴细胞（细胞毒性/抑制性T细胞）被隔离在上皮样结节的周边，而辅助诱导性CD4 + T淋巴细胞则分布在整个浸润区域。表皮中CD1 +朗格汉斯细胞增加，在真皮中它们主要定位于肉芽肿周围的包膜中。接种麻风分枝杆菌 - 卡介苗后产生的大多数真皮浸润物表达HLA - DR抗原。同样，大多数角质形成细胞对这种MHC抗原也呈阳性。对卡介苗的肉芽肿反应与接种麻风分枝杆菌 - 卡介苗混合物相似，然而抗酸杆菌仍然存在。接种麻风分枝杆菌产生了巨噬细胞肉芽肿，且杆菌未清除，这类似于瘤型麻风肉芽肿。
