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体外共培养诱导人羊膜间充质干细胞向人前交叉韧带成纤维细胞分化。

Differentiation of Human Amniotic Mesenchymal Stem Cells into Human Anterior Cruciate Ligament Fibroblast Cells by In Vitro Coculture.

机构信息

The First Department of Orthopedics, The Affiliated Hospital of Zunyi Medical College, Zunyi, Guizhou 563000, China.

The Department of Orthopedics, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.

出版信息

Biomed Res Int. 2017;2017:7360354. doi: 10.1155/2017/7360354. Epub 2017 Sep 20.

DOI:10.1155/2017/7360354
PMID:29085840
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5632453/
Abstract

Anterior cruciate ligament injuries are common in humans, though cellular components of the knee have little regenerative or proliferation potential. This study investigated the differentiation of human amnion-derived mesenchymal stem cells (hAMSCs) into human anterior cruciate ligament fibroblasts (hACLFs) in vitro through induction with bFGF and TGF-1 with coculture systems. Groups A and B comprised hAMSCs at the 3rd passage cultured with and without bFGF and TGF-1, respectively; Groups C and D consisted of hAMSCs and hACLFs in monolayer coculture with and without bFGF and TGF-1, respectively; Groups E and F were composed of hAMSCs and hACLFs in Transwell coculture with and without bFGF and TGF-1, respectively. Cell morphology and proliferation were recorded. Protein expression and relative mRNA expression were evaluated in each group. Cell proliferation was significantly higher in the induced groups than in the noninduced groups. Protein expression increased over time with the highest expression observed in Group E. mRNA levels were significantly higher in Group E than in other groups. This study is the first to demonstrate the use of the Transwell coculture system for this purpose, and hAMSCs were successfully differentiated into hACLFs. Thus, hAMSCs may be a superior choice for hACLF differentiation via Transwell coculture.

摘要

前交叉韧带损伤在人类中很常见，尽管膝关节的细胞成分再生或增殖的潜力很小。本研究通过 bFGF 和 TGF-1 诱导以及共培养系统，体外研究了人羊膜间充质干细胞（hAMSCs）向人前交叉韧带成纤维细胞（hACLFs）的分化。A 组和 B 组分别为第 3 代培养的 hAMSCs，分别加入和不加入 bFGF 和 TGF-1；C 组和 D 组分别为单层共培养的 hAMSCs 和 hACLFs，分别加入和不加入 bFGF 和 TGF-1；E 组和 F 组分别为 Transwell 共培养的 hAMSCs 和 hACLFs，分别加入和不加入 bFGF 和 TGF-1。记录细胞形态和增殖情况。评估各组的蛋白表达和相对 mRNA 表达。诱导组的细胞增殖明显高于非诱导组。蛋白表达随时间增加，E 组表达最高。E 组的 mRNA 水平明显高于其他组。本研究首次证明了 Transwell 共培养系统可用于此目的，hAMSCs 成功分化为 hACLFs。因此，hAMSCs 可能是 Transwell 共培养分化 hACLF 的更好选择。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5634/5632453/81df1567839e/BMRI2017-7360354.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5634/5632453/6d58d2668878/BMRI2017-7360354.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5634/5632453/a0e2278cb217/BMRI2017-7360354.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5634/5632453/5f2a4f0d2866/BMRI2017-7360354.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5634/5632453/1c0de3c750c7/BMRI2017-7360354.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5634/5632453/ed99efff2109/BMRI2017-7360354.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5634/5632453/8e9e8ee5fef6/BMRI2017-7360354.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5634/5632453/b75dd37c105e/BMRI2017-7360354.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5634/5632453/81df1567839e/BMRI2017-7360354.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5634/5632453/6d58d2668878/BMRI2017-7360354.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5634/5632453/a0e2278cb217/BMRI2017-7360354.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5634/5632453/5f2a4f0d2866/BMRI2017-7360354.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5634/5632453/1c0de3c750c7/BMRI2017-7360354.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5634/5632453/ed99efff2109/BMRI2017-7360354.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5634/5632453/8e9e8ee5fef6/BMRI2017-7360354.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5634/5632453/b75dd37c105e/BMRI2017-7360354.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5634/5632453/81df1567839e/BMRI2017-7360354.008.jpg

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