Coombe Women and Infants University Hospital, Department of Pathology, Dublin, Ireland.
Trinity College, School of Medicine, Department of Histopathology and Morbid Anatomy, Dublin, Ireland.
J Biomed Opt. 2017 Oct;22(10):1-10. doi: 10.1117/1.JBO.22.10.105008.
It is widely accepted that cervical screening has significantly reduced the incidence of cervical cancer worldwide. The primary screening test for cervical cancer is the Papanicolaou (Pap) test, which has extremely variable specificity and sensitivity. There is an unmet clinical need for methods to aid clinicians in the early detection of cervical precancer. Raman spectroscopy is a label-free objective method that can provide a biochemical fingerprint of a given sample. Compared with studies on infrared spectroscopy, relatively few Raman spectroscopy studies have been carried out to date on cervical cytology. The aim of this study was to define the Raman spectral signatures of cervical exfoliated cells present in liquid-based cytology Pap test specimens and to compare the signature of high-grade dysplastic cells to each of the normal cell types. Raman spectra were recorded from single exfoliated cells and subjected to multivariate statistical analysis. The study demonstrated that Raman spectroscopy can identify biochemical signatures associated with the most common cell types seen in liquid-based cytology samples; superficial, intermediate, and parabasal cells. In addition, biochemical changes associated with high-grade dysplasia could be identified suggesting that Raman spectroscopy could be used to aid current cervical screening tests.
人们普遍认为，宫颈筛查显著降低了全球范围内宫颈癌的发病率。宫颈癌的主要筛查试验是巴氏涂片（Pap）检查，但其特异性和敏感性差异极大。临床需要新的方法来帮助医生早期发现宫颈癌前病变。拉曼光谱是一种无标记的客观方法，可以提供给定样本的生化指纹。与红外光谱研究相比，迄今为止，对宫颈细胞学的拉曼光谱研究相对较少。本研究旨在确定液基细胞学巴氏涂片标本中宫颈脱落细胞的拉曼光谱特征，并比较高级别异型细胞的特征与每种正常细胞类型的特征。从单个脱落细胞中记录拉曼光谱，并进行多元统计分析。研究表明，拉曼光谱可以识别与液基细胞学样本中最常见的细胞类型（表层、中层和副基底细胞）相关的生化特征。此外，还可以识别与高级别异型相关的生化变化，表明拉曼光谱可用于辅助当前的宫颈癌筛查试验。
