1,2,4-三唑辅助的肽-N-酰基-N'-甲基苯并咪唑啉酮与半胱氨酰肽之间的天然化学连接
1,2,4-Triazole-aided native chemical ligation between peptide-N-acyl-N'-methyl-benzimidazolinone and cysteinyl peptide.
作者信息
Sakamoto Ken, Tsuda Shugo, Nishio Hideki, Yoshiya Taku
机构信息
Peptide Institute, Inc., Ibaraki-shi, Osaka 567-0085, Japan.
出版信息
Chem Commun (Camb). 2017 Nov 9;53(90):12236-12239. doi: 10.1039/c7cc07817j.
PMID:29086777
Abstract
1,2,4-Triazole facilitated native chemical ligation (NCL) between peptide-MeNbz (MeNbz: N-acyl-N'-methyl-benzimidazolinone) and a cysteinyl peptide in the absence of thiol additives. The method enabled one-pot desulfurization and iodine oxidation after NCL. Additionally, the direct isolation of the target peptide from the NCL reaction mixture with an activated thiopropyl-Sepharose resin was achieved.
摘要
1,2,4-三唑在无硫醇添加剂的情况下促进了肽-MeNbz（MeNbz：N-酰基-N'-甲基苯并咪唑啉酮）与半胱氨酰肽之间的天然化学连接（NCL）。该方法能够在NCL后进行一锅法脱硫和碘氧化。此外，还实现了用活化的硫丙基-琼脂糖树脂从NCL反应混合物中直接分离目标肽。
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