Ye Wenyun, Zhang Cheng, Xu Na, Sun Yan, Ma Lei, Shen Bo, Zhou Dan, Zhu Changliang
Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu, China.
Arch Insect Biochem Physiol. 2017 Dec;96(4). doi: 10.1002/arch.21428. Epub 2017 Oct 31.
Carbonic anhydrases (CAs) are metabolic enzymes that regulate the physiological equilibrium in a variety of organisms. In this study, we investigated the function of CA II in Culex pipiens pallens using real-time quantitative polymerase chain reaction and double-stranded RNA injection. CA II transcripts were more abundant in the deltamethrin-susceptible strain than the deltamethrin-resistant strain. The activities of metabolic enzymes increased when the CA II expression was silenced. These findings suggest CA II regulates deltamethrin resistance by altering metabolic enzyme activity, and could serve as a potential genetic marker for monitoring deltamethrin resistance in mosquitoes.
碳酸酐酶（CAs）是调节多种生物体生理平衡的代谢酶。在本研究中，我们使用实时定量聚合酶链反应和双链RNA注射研究了淡色库蚊中CA II的功能。与溴氰菊酯抗性品系相比，CA II转录本在溴氰菊酯敏感品系中更为丰富。当CA II表达沉默时，代谢酶的活性增加。这些发现表明，CA II通过改变代谢酶活性来调节溴氰菊酯抗性，并且可以作为监测蚊子溴氰菊酯抗性的潜在遗传标记。
