Knez Judita, Lakota Katja, Božič Nina, Okrajšek Renata, Cauwenberghs Nicholas, Thijs Lutgarde, Kneževič Ivan, Vrtovec Bojan, Tomšič Matija, Čučnik Saša, Sodin-Šemrl Snežna, Kuznetsova Tatiana, Brguljan-Hitij Jana
1 Division of Internal Medicine, Department of Hypertension, University Medical Centre Ljubljana , Ljubljana, Slovenia .
2 Research Unit Hypertension and Cardiovascular Epidemiology, KU Leuven Department of Cardiovascular Sciences, University of Leuven , Leuven, Belgium .
Genet Test Mol Biomarkers. 2017 Dec;21(12):736-741. doi: 10.1089/gtmb.2017.0099. Epub 2017 Oct 31.
BACKGROUND/OBJECTIVES: Heart failure (HF) is associated with disturbances in mitochondrial energy production. This mitochondrial dysfunction is reflected by depletion of mitochondrial DNA (mtDNA) in different tissues. Our aims were to determine if there was a correlation between mtDNA content measured in myocardial tissue and the easily accessible peripheral blood cells of patients with non-ischemic HF; and to determine if there was a correlation between myocardial mtDNA and left ventricular (LV) ejection fraction.
We prospectively collected paired myocardial tissue and peripheral blood samples from 13 consecutive end-stage non-ischemic HF patients undergoing cardiac transplantation. mtDNA content was assessed with real-time quantitative PCR by calculating the relative ratio of two specific mitochondrial sequences and one nuclear control gene sequence.
HF patients with lower myocardial mtDNA content had a significantly lower LV ejection fraction (r = 0.65, p = 0.016). Peripheral blood mtDNA content correlated positively with right ventricular myocardial mtDNA content (r = 0.63, p = 0.021). We also observed that averaged myocardial DNA content tended to correlate with peripheral blood mtDNA content (r = 0.53, p = 0.061).
In non-ischemic HF patients, myocardial mtDNA content is positively correlated with peripheral blood mtDNA content and LV function as assessed by echocardiography.
背景/目的：心力衰竭（HF）与线粒体能量产生紊乱有关。这种线粒体功能障碍表现为不同组织中线粒体DNA（mtDNA）的耗竭。我们的目的是确定在非缺血性HF患者的心肌组织中测得的mtDNA含量与易于获取的外周血细胞中的mtDNA含量之间是否存在相关性；并确定心肌mtDNA与左心室（LV）射血分数之间是否存在相关性。
我们前瞻性地收集了13例连续接受心脏移植的终末期非缺血性HF患者的配对心肌组织和外周血样本。通过计算两个特定线粒体序列与一个核对照基因序列的相对比率，采用实时定量PCR评估mtDNA含量。
心肌mtDNA含量较低的HF患者的LV射血分数显著较低（r = 0.65，p = 0.016）。外周血mtDNA含量与右心室心肌mtDNA含量呈正相关（r = 0.63，p = 0.021）。我们还观察到平均心肌DNA含量倾向于与外周血mtDNA含量相关（r = 0.53，p = 0.061）。
在非缺血性HF患者中，心肌mtDNA含量与外周血mtDNA含量以及经超声心动图评估的LV功能呈正相关。
