• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

利用生物层干涉生物传感器技术进行疫苗研究与开发的策略。

Strategies Using Bio-Layer Interferometry Biosensor Technology for Vaccine Research and Development.

机构信息

Pall Fortebio, 47661 Fremont Boulevard, Fremont, CA 94538, USA.

出版信息

Biosensors (Basel). 2017 Oct 31;7(4):49. doi: 10.3390/bios7040049.

DOI:10.3390/bios7040049
PMID:29088096
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5746772/
Abstract

Bio-layer interferometry (BLI) real-time, label-free technology has greatly contributed to advances in vaccine research and development. BLI Octet platforms offer high-throughput, ease of use, reliability, and high precision analysis when compared with common labeling techniques. Many different strategies have been used to immobilize the pathogen or host molecules on BLI biosensors for real-time kinetics and affinity analysis, quantification, or high-throughput titer. These strategies can be used in multiple applications and shed light onto the structural and functional aspects molecules play during pathogen-host interactions. They also provide crucial information on how to achieve protection. This review summarizes some key BLI strategies used in human vaccine research and development.

摘要

生物层干涉测量（BLI）实时、无标记技术极大地促进了疫苗研究与开发的进展。与常见的标记技术相比，BLI Octet 平台具有高通量、易于使用、可靠性和高精度分析等优点。为了实时动力学和亲和力分析、定量或高通量滴度，已经使用了许多不同的策略将病原体或宿主分子固定在 BLI 生物传感器上。这些策略可用于多种应用，并揭示了分子在病原体-宿主相互作用过程中的结构和功能方面的作用。它们还提供了有关如何实现保护的重要信息。本文综述了用于人类疫苗研究与开发的一些关键 BLI 策略。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3be7/5746772/471be2f71e31/biosensors-07-00049-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3be7/5746772/9308e6d5aff6/biosensors-07-00049-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3be7/5746772/a752b17fae2f/biosensors-07-00049-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3be7/5746772/e7b54733c2ee/biosensors-07-00049-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3be7/5746772/27862bb98e2b/biosensors-07-00049-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3be7/5746772/bd07c10d56f0/biosensors-07-00049-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3be7/5746772/f1fb172908c3/biosensors-07-00049-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3be7/5746772/5e93ddb8aebd/biosensors-07-00049-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3be7/5746772/471be2f71e31/biosensors-07-00049-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3be7/5746772/9308e6d5aff6/biosensors-07-00049-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3be7/5746772/a752b17fae2f/biosensors-07-00049-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3be7/5746772/e7b54733c2ee/biosensors-07-00049-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3be7/5746772/27862bb98e2b/biosensors-07-00049-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3be7/5746772/bd07c10d56f0/biosensors-07-00049-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3be7/5746772/f1fb172908c3/biosensors-07-00049-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3be7/5746772/5e93ddb8aebd/biosensors-07-00049-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3be7/5746772/471be2f71e31/biosensors-07-00049-g008.jpg

相似文献

1
Strategies Using Bio-Layer Interferometry Biosensor Technology for Vaccine Research and Development.利用生物层干涉生物传感器技术进行疫苗研究与开发的策略。
Biosensors (Basel). 2017 Oct 31;7(4):49. doi: 10.3390/bios7040049.
2
Label-free detection of biomolecular interactions using BioLayer interferometry for kinetic characterization.使用生物层干涉术进行动力学表征的生物分子相互作用的无标记检测。
Comb Chem High Throughput Screen. 2009 Sep;12(8):791-800. doi: 10.2174/138620709789104915.
3
An approach for liposome immobilization using sterically stabilized micelles (SSMs) as a precursor for bio-layer interferometry-based interaction studies.一种使用空间稳定胶束（SSM）作为基于生物层干涉术的相互作用研究的前体来固定脂质体的方法。
Colloids Surf B Biointerfaces. 2017 Jun 1;154:186-194. doi: 10.1016/j.colsurfb.2017.03.015. Epub 2017 Mar 12.
4
Designing binding kinetic assay on the bio-layer interferometry (BLI) biosensor to characterize antibody-antigen interactions.在生物层干涉术（BLI）生物传感器上设计结合动力学测定法以表征抗体 - 抗原相互作用。
Anal Biochem. 2017 Nov 1;536:16-31. doi: 10.1016/j.ab.2017.08.002. Epub 2017 Aug 10.
5
A novel label-free cell-based assay technology using biolayer interferometry.一种使用生物层干涉测量法的新型无标记细胞检测技术。
Biosens Bioelectron. 2017 Jan 15;87:388-395. doi: 10.1016/j.bios.2016.08.095. Epub 2016 Aug 28.
6
An Experimental Framework for Developing Point-of-Need Biosensors: Connecting Bio-Layer Interferometry and Electrochemical Impedance Spectroscopy.用于开发即时生物传感器的实验框架：生物层干涉法和电化学阻抗谱的连接。
Biosensors (Basel). 2022 Oct 29;12(11):938. doi: 10.3390/bios12110938.
7
Practical quantitative and kinetic applications of bio-layer interferometry for toxicokinetic analysis of a monoclonal antibody therapeutic.用于单克隆抗体治疗药物毒代动力学分析的生物层干涉法的实用定量和动力学应用。
J Immunol Methods. 2012 May 31;379(1-2):30-41. doi: 10.1016/j.jim.2012.02.017. Epub 2012 Mar 4.
8
Development of a Bio-Layer Interferometry-Based Protease Assay Using HIV-1 Protease as a Model.基于生物层干涉法的 HIV-1 蛋白酶模型蛋白酶分析方法的建立。
Viruses. 2021 Jun 21;13(6):1183. doi: 10.3390/v13061183.
9
Label-free kinetic analysis of an antibody-antigen interaction using biolayer interferometry.使用生物膜干涉术对抗体 - 抗原相互作用进行无标记动力学分析。
Methods Mol Biol. 2015;1278:165-82. doi: 10.1007/978-1-4939-2425-7_10.
10
Application of Bio-Layer Interferometry for the analysis of protein/liposome interactions.应用生物层干涉技术分析蛋白/脂质体相互作用。
J Pharm Biomed Anal. 2013 Jan;72:150-4. doi: 10.1016/j.jpba.2012.10.008. Epub 2012 Oct 13.

引用本文的文献

1
Discovery of Small-Molecule PD-L1 Inhibitors via Virtual Screening and Their Immune-Mediated Anti-Tumor Effects.通过虚拟筛选发现小分子PD-L1抑制剂及其免疫介导的抗肿瘤作用。
Pharmaceuticals (Basel). 2025 Aug 15;18(8):1209. doi: 10.3390/ph18081209.
2
Characterization of antiviral compounds using Bio-Layer Interferometry.使用生物层干涉术对抗病毒化合物进行表征。
bioRxiv. 2025 Jul 24:2025.07.24.662752. doi: 10.1101/2025.07.24.662752.
3
An Overview of Epitopes and Methods for Antibody Epitope Mapping.表位及抗体表位图谱绘制方法概述

本文引用的文献

1
Designing binding kinetic assay on the bio-layer interferometry (BLI) biosensor to characterize antibody-antigen interactions.在生物层干涉术（BLI）生物传感器上设计结合动力学测定法以表征抗体 - 抗原相互作用。
Anal Biochem. 2017 Nov 1;536:16-31. doi: 10.1016/j.ab.2017.08.002. Epub 2017 Aug 10.
2
Evaluation of multiplex assay platforms for detection of influenza hemagglutinin subtype specific antibody responses.用于检测流感血凝素亚型特异性抗体反应的多重检测平台评估。
J Virol Methods. 2017 May;243:61-67. doi: 10.1016/j.jviromet.2017.01.008. Epub 2017 Jan 17.
3
Anti-MrkA Monoclonal Antibodies Reveal Distinct Structural and Antigenic Features of MrkA.
Methods Mol Biol. 2025;2937:1-11. doi: 10.1007/978-1-0716-4591-8_1.
4
Development of a frontal analysis capillary electrophoresis coupled with time-of-flight mass spectrometry for determining the equilibrium dissociation constant between cyclophilin A and cyclosporin A.用于测定亲环蛋白A与环孢素A之间平衡解离常数的前沿分析毛细管电泳结合飞行时间质谱法的开发。
Anal Sci. 2025 May 19. doi: 10.1007/s44211-025-00790-0.
5
Modular Nanoparticle Platform for Solution-Phase Optical Sensing of Protein-Protein Interactions.用于蛋白质-蛋白质相互作用溶液相光学传感的模块化纳米颗粒平台。
ACS Appl Opt Mater. 2025 Mar 15;3(3):676-688. doi: 10.1021/acsaom.4c00486. eCollection 2025 Mar 28.
6
Therapeutic Potential of Qilianxiaopi Formula: Targeting ADAM17-Mediated Chronic Inflammation in Atrophic Gastritis.芪连消痞方的治疗潜力：针对萎缩性胃炎中ADAM17介导的慢性炎症
Pharmaceuticals (Basel). 2025 Mar 19;18(3):435. doi: 10.3390/ph18030435.
7
A humanized antibody against mucormycosis targets angioinvasion and augments the host immune response.一种抗毛霉菌病的人源化抗体靶向血管侵袭并增强宿主免疫反应。
Sci Transl Med. 2025 Mar 12;17(789):eads7369. doi: 10.1126/scitranslmed.ads7369.
8
Functional Activity and Binding Specificity of Small Ankyrin Repeat Proteins Called Ankyrons Against SARS-CoV-2 Variants.名为锚蛋白的小锚蛋白重复序列蛋白对新冠病毒变种的功能活性和结合特异性
AAPS J. 2025 Mar 11;27(2):58. doi: 10.1208/s12248-025-01043-8.
9
Production and Bioseparation Applications of Polyhydroxyalkanoate Nano-Granules Functionalized with Streptavidin.用链霉亲和素功能化的聚羟基脂肪酸酯纳米颗粒的生产及生物分离应用
Microorganisms. 2025 Feb 1;13(2):312. doi: 10.3390/microorganisms13020312.
10
Focal Molography Allows for Affinity and Concentration Measurements of Proteins in Complex Matrices with High Accuracy.聚焦全息术可高精度地对复杂基质中的蛋白质进行亲和力和浓度测量。
Biosensors (Basel). 2025 Jan 22;15(2):66. doi: 10.3390/bios15020066.
抗MrkA单克隆抗体揭示了MrkA独特的结构和抗原特征。
PLoS One. 2017 Jan 20;12(1):e0170529. doi: 10.1371/journal.pone.0170529. eCollection 2017.
4
Generation of a highly diverse panel of antagonistic chicken monoclonal antibodies against the GIP receptor.针对GIP受体生成高度多样化的拮抗鸡单克隆抗体库。
MAbs. 2017 Apr;9(3):536-549. doi: 10.1080/19420862.2016.1276683. Epub 2017 Jan 5.
5
A Randomized, Controlled Trial of ZMapp for Ebola Virus Infection.ZMapp治疗埃博拉病毒感染的随机对照试验。
N Engl J Med. 2016 Oct 13;375(15):1448-1456. doi: 10.1056/NEJMoa1604330.
6
Structural basis of potent Zika-dengue virus antibody cross-neutralization.强效寨卡-登革热病毒抗体交叉中和的结构基础。
Nature. 2016 Aug 4;536(7614):48-53. doi: 10.1038/nature18938. Epub 2016 Jun 23.
7
Isolation of potent neutralizing antibodies from a survivor of the 2014 Ebola virus outbreak.从2014年埃博拉病毒疫情的一名幸存者体内分离出强效中和抗体。
Science. 2016 Mar 4;351(6277):1078-83. doi: 10.1126/science.aad5788. Epub 2016 Feb 18.
8
Antibody therapeutics for Ebola virus disease.用于埃博拉病毒病的抗体疗法。
Curr Opin Virol. 2016 Apr;17:45-49. doi: 10.1016/j.coviro.2016.01.006. Epub 2016 Jan 27.
9
Trichomonas vaginalis Lipophosphoglycan Exploits Binding to Galectin-1 and -3 to Modulate Epithelial Immunity.阴道毛滴虫脂磷壁酸聚糖通过与半乳糖凝集素-1和-3结合来调节上皮免疫。
J Biol Chem. 2016 Jan 8;291(2):998-1013. doi: 10.1074/jbc.M115.651497. Epub 2015 Nov 20.
10
Bispecific Antibodies Targeting Different Epitopes on the HIV-1 Envelope Exhibit Broad and Potent Neutralization.靶向HIV-1包膜上不同表位的双特异性抗体表现出广泛而有效的中和作用。
J Virol. 2015 Dec;89(24):12501-12. doi: 10.1128/JVI.02097-15. Epub 2015 Oct 7.