Canine distemper virus infectivity of various blood fractions for central nervous system vasculature.

In order to determine the infectivity of various viremic blood fractions for central nervous system (CNS) endothelia, viremic plasma, platelets and mononuclear cells were prepared from canine distemper virus (CDV)-infected dogs and infused into the right carotid arteries of CDV-naive gnotobiotic dogs. All blood fractions were infectious for endothelia as determined by indirect immunofluorescence examination for viral antigen in recipients. Virus-positive platelets, even though possessing only trace amounts (1.0 x 10(1) TCID50/ml) of in vitro titratable virus, were the most effective fraction for infection of vascular endothelium. These data confirm the important role of vascular endothelia in establishing CNS infection in this disease and implicate virus-positive platelets and leukocytes in the initiation of this phenomenon.