一种用于恶性疟原虫的多功能、高通量、基于珠粒的吞噬作用检测方法。
A versatile, high through-put, bead-based phagocytosis assay for Plasmodium falciparum.
机构信息
Department of Tropical Medicine, Medical Microbiology and Pharmacology, John A. Burns School of Medicine, University of Hawaii at Manoa, Hawaii, USA.
Centre for Medical Parasitology, Department of Immunology and Microbiology, University of Copenhagen, Copenhagen, Denmark.
出版信息
Sci Rep. 2017 Oct 31;7(1):14705. doi: 10.1038/s41598-017-13900-4.
Antibody-mediated phagocytosis is an important immune effector mechanism against Plasmodium falciparum-infected erythrocytes (IE); however, current phagocytosis assays use IE collected from infected individuals or from in vitro cultures of P. falciparum, making them prone to high variation. A simple, high-throughput flow cytometric assay was developed that uses THP-1 cells and fluorescent beads covalently-coupled with the malarial antigen VAR2CSA. The assay is highly repeatable, provides both the overall percent phagocytosis and semi-quantitates the number of antigen-coupled beads internalized.
抗体介导的吞噬作用是一种针对恶性疟原虫感染红细胞（IE）的重要免疫效应机制；然而，目前的吞噬作用检测方法使用来自感染个体或恶性疟原虫体外培养物的 IE，这使得它们容易产生高度变异。本研究开发了一种简单、高通量的流式细胞术检测方法，该方法使用 THP-1 细胞和荧光珠共价偶联恶性疟原虫抗原 VAR2CSA。该检测方法重复性高，既能提供总体吞噬百分比，又能半定量检测内化的抗原偶联珠数量。
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