Programa de Pós-Graduação em Biotecnologia, Universidade Federal do Amazonas (UFAM), Manaus 69067-005, Brazil.
Instituto Leônidas e Maria Deane (ILMD), Fundação Oswaldo Cruz (FIOCRUZ), Manaus 69057-070, Brazil.
Exp Biol Med (Maywood). 2022 Oct;247(20):1852-1861. doi: 10.1177/15353702221113856. Epub 2022 Aug 16.
Microsphere-based flow cytometry is a highly sensitive emerging technology for specific detection and clinical analysis of antigens, antibodies, and nucleic acids of interest. In this review, studies that focused on the application of flow cytometry as a viable alternative for the investigation of infectious diseases were analyzed. Many of the studies involve research aimed at epidemiological surveillance, vaccine candidates and early diagnosis, non-infectious diseases, specifically cancer, and emphasize the simultaneous detection of biomarkers for early diagnosis, with accurate results in a non-invasive approach. The possibility of carrying out multiplexed assays affords this technique high versatility and performance, which is evidenced in a series of clinical studies that have verified the ability to detect several molecules in low concentrations and with minimal sample volume. As such, we demonstrate that microsphere-based flow cytometry presents itself as a promising technique that can be adopted as a fundamental element in the development of new diagnostic methods for a number of diseases.
基于微球的流式细胞术是一种用于检测和分析特定抗原、抗体和核酸的新兴技术，具有高度敏感性。本综述分析了将流式细胞术作为一种可行的传染病研究替代方法的研究。许多研究涉及旨在进行流行病学监测、疫苗候选物和早期诊断的研究，也涉及非传染性疾病（特别是癌症），强调通过非侵入性方法进行早期诊断的生物标志物的同时检测，并获得准确的结果。多重分析的可能性使该技术具有高度的多功能性和性能，一系列临床研究已经证明了该技术能够以最小的样本量检测低浓度的多种分子。因此，我们证明了基于微球的流式细胞术是一种很有前途的技术，可以作为开发多种疾病新诊断方法的基本要素。
