Jung So-Youn, Kim Kyoung-Min, Cho Suin, Lim Sehyun, Lim Chiyeon, Kim Young Kyun
College of Korean Medicine, Dong-Eui University, Yangjeong-ro, Busanjin-gu, Busan, 47227 Republic of Korea.
School of Korean Medicine, Pusan National University, Yangsan, Gyeongnam 50612 Republic of Korea.
Chin Med. 2017 Oct 24;12:30. doi: 10.1186/s13020-017-0151-z. eCollection 2017.
Stroke is the second most common cause of death and may result in various disabilities; thus, identification of neuroprotective therapeutic agents is important. Peucedani Radix (PR), the root of , is a well-known remedy for damp and phlegm in Korean medicine and has also been shown to exert antioxidant and anti-inflammatory activities. This study was performed to investigate the mechanism underlying the anti-inflammatory effect of methanol extract of PR (PRex) on cerebral ischemic injury.
C57BL/6 male mice were orally administered PRex (20, 60, or 200 mg/kg) at 2 days, 1 day, and 1 h prior to middle cerebral artery occlusion (MCAO). Twenty-four hours after MCAO, the infarct volume was measured and the neurological deficit score was assessed. The inflammatory-related substances in the ipsilateral hemisphere were determined by western blotting, DCFH-DA assay, TBARS assay, and ELISA.
PRex pretreatment significantly decreased the infarct volume at 24 h after MCAO. Moreover, PRex effectively suppressed the expression of iNOS, ROS, MDA, and pro-inflammatory cytokines, such as IL-1β and TNF-α, in brain tissue of mice with MCAO-induced brain injury.
PRex protected neurons from ischemic brain injury in mice through its antioxidant and anti-inflammatory activities. Our results suggested that PR could be a promising candidate in the therapy of ischemia-induced brain damage.
中风是第二大常见死因，可能导致各种残疾；因此，确定神经保护治疗药物很重要。前胡（PR），即紫花前胡的根，是韩国医学中治疗痰湿的著名药物，也已显示出具有抗氧化和抗炎活性。本研究旨在探讨前胡甲醇提取物（PRex）对脑缺血损伤抗炎作用的潜在机制。
在大脑中动脉闭塞（MCAO）前2天、1天和1小时，对C57BL/6雄性小鼠口服给予PRex（20、60或200mg/kg）。MCAO后24小时，测量梗死体积并评估神经功能缺损评分。通过蛋白质免疫印迹法、DCFH-DA检测、TBARS检测和酶联免疫吸附测定法测定同侧半球的炎症相关物质。
PRex预处理显著降低了MCAO后24小时的梗死体积。此外，PRex有效抑制了MCAO诱导的脑损伤小鼠脑组织中诱导型一氧化氮合酶、活性氧、丙二醛以及促炎细胞因子如白细胞介素-1β和肿瘤坏死因子-α的表达。
PRex通过其抗氧化和抗炎活性保护小鼠神经元免受缺血性脑损伤。我们的结果表明，前胡可能是治疗缺血性脑损伤的一个有前景的候选药物。
