Poursani Ensieh M, Mehravar Majid, Shahryari Alireza, Mowla Seyed Javad, Mohammad Soltani Bahram
Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.
Avicenna J Med Biotechnol. 2017 Oct-Dec;9(4):201-204.
The human gene, responsible for pluripotency and self-renewal of Embryonic Stem (ES) and Embryonic Carcinoma (EC) cells, can generate several transcripts (OCT4A, OCT4B-variant 2, OCT4B-variant 3, OCT4B-variant 5, OCT4B1, OCT4 B2 and OCT4B3) by alternative splicing and alternative promoters. OCT4A that is responsible for ES and EC cell stemness properties is transcribed from a promoter upstream of Exon1a in those cells. The OCT4B group variants (OCT4B-variant2, OCT4B-variant3, OCT4B-variant5, OCT4B1, OCT4B2 and OCT4B3) are transcribed from a different promoter located in intron 1 and some of them respond to the cell stresses, but cannot sustain the ES/EC cell self-renewal. However, the exact function of OCT4B group variants is still unclear.
In the present study, we employed RT-PCR and sequencing approaches to explore different forms of transcripts.
Our data revealed that the OCT4B group variants (OCT4B-variant2, OCT4 B-variant3, OCT4B1, OCT4B2 and OCT4B3) have longer 5' UTR in the human bladder carcinoma cell line of 5637.
These variants undergo alternative splicing in their 5' UTR which might exert regulatory roles in transcription and translation mechanisms.
人类基因负责胚胎干细胞（ES）和胚胎癌细胞（EC）的多能性和自我更新，可通过可变剪接和可变启动子产生多种转录本（OCT4A、OCT4B变体2、OCT4B变体3、OCT4B变体5、OCT4B1、OCT4 B2和OCT4B3）。负责ES和EC细胞干性特性的OCT4A在这些细胞中从外显子1a上游的启动子转录而来。OCT4B组变体（OCT4B变体2、OCT4B变体3、OCT4B变体5、OCT4B1、OCT4B2和OCT4B3）从位于内含子1中的不同启动子转录而来，其中一些对细胞应激有反应，但不能维持ES/EC细胞的自我更新。然而，OCT4B组变体的确切功能仍不清楚。
在本研究中，我们采用逆转录聚合酶链反应（RT-PCR）和测序方法来探索不同形式的转录本。
我们的数据显示，在人膀胱癌细胞系5637中，OCT4B组变体（OCT4B变体2、OCT4 B变体3、OCT4B1、OCT4B2和OCT4B3）具有更长的5'非翻译区（UTR）。
这些变体在其5'UTR中经历可变剪接，这可能在转录和翻译机制中发挥调节作用。
