Wang Tao, Chen Lu, Guo Qiao-Sheng, Zhang Xiao-Ming, Song Ling-Shan
Institute of Chinese Medicinal Materials, Nanjing Agricultural University, Nanjing 210095, China.
Zhongguo Zhong Yao Za Zhi. 2017 May;42(10):1847-1852. doi: 10.19540/j.cnki.cjcmm.2017.0075.
The study is aimed at determine the content of anthocyanins and expressions of relative genes and activity of relative enzymes. The effects of flood stress on anthocyanins synthesis with relative genes and enzymes of Chrysanthemum morifolium cv. 'Hangju' were analyzed. The expression of CHS and the content of CHS presented the trend of first rising and after downward with the increase of flowering degree. The content of anthocyanins, the expression of DFR and the activity of DFR presented the trend of first downward and after rising with the increase of flowering degree. There was a positive correlation among anthocyanins,DFR gene and DFR. However there was no significant correlation among anthocyanins,CHS gene and CHS. Flood stress has significant effects on anthocyanins synthesis with relative genes and enzymes of Ch. morifolium cv. 'Hangju',but don't change the patterns of genes expression and anthocyanins and enzymes accumulation. DFR and DFR are the key gene and key enzyme of anthocyanins synthesis.
本研究旨在测定花青素含量、相关基因的表达以及相关酶的活性。分析了淹水胁迫对菊花品种‘杭菊’花青素合成及其相关基因和酶的影响。随着开花程度的增加，CHS的表达和含量呈现先上升后下降的趋势。随着开花程度的增加，花青素含量、DFR的表达和DFR的活性呈现先下降后上升的趋势。花青素、DFR基因和DFR之间存在正相关。然而，花青素、CHS基因和CHS之间没有显著相关性。淹水胁迫对‘杭菊’花青素合成及其相关基因和酶有显著影响，但不改变基因表达模式以及花青素和酶的积累模式。DFR和DFR是花青素合成的关键基因和关键酶。
