吲哚美辛通过下调小眼畸形相关转录因子（Mitf）mRNA转录来抑制黑色素生成。
Indomethacin inhibits melanogenesis via down-regulation of Mitf mRNA transcription.
作者信息
Sato Kazuomi, Takei Masahiro, Iyota Ray, Muraoka Yoshimasa, Nagashima Marika, Yoshimura Yoshitaka
机构信息
a Department of Agri-Production Sciences, College of Agriculture , Tamagawa University , Machida, Tokyo , Japan.
c Graduate School of Agriculture , Tamagawa University , Tamagawa-gakuen, Machida, Tokyo , Japan.
出版信息
Biosci Biotechnol Biochem. 2017 Dec;81(12):2307-2313. doi: 10.1080/09168451.2017.1394812. Epub 2017 Nov 1.
Non-steroidal anti-inflammatory drugs (NSAIDs) exhibit several divergent biological effects. In this study, we investigated the effect of indomethacin on melanin synthesis using B16F1 melanoma cells. Indomethacin inhibited α-melanocyte stimulating hormone (α-MSH)-enhanced melanin synthesis in a dose-dependent manner. Western blotting analysis revealed that indomethacin significantly suppressed tyrosinase and Mitf protein levels. In a luciferase reporter assay, we found that indomethacin reduced tyrosinase promoter activity. Moreover, real-time RT-PCR analysis showed that indomethacin lowered mRNA levels of melanogenic genes, including Mitf. Together, our findings indicate that indomethacin inhibits melanogenesis via the suppression of Mitf transcription.
非甾体抗炎药（NSAIDs）具有多种不同的生物学效应。在本研究中，我们使用B16F1黑色素瘤细胞研究了吲哚美辛对黑色素合成的影响。吲哚美辛以剂量依赖性方式抑制α-黑素细胞刺激激素（α-MSH）增强的黑色素合成。蛋白质印迹分析显示，吲哚美辛显著抑制酪氨酸酶和小眼畸形相关转录因子（Mitf）蛋白水平。在荧光素酶报告基因检测中，我们发现吲哚美辛降低了酪氨酸酶启动子活性。此外，实时逆转录聚合酶链反应（RT-PCR）分析表明，吲哚美辛降低了包括Mitf在内的黑色素生成基因的mRNA水平。总之，我们的研究结果表明，吲哚美辛通过抑制Mitf转录来抑制黑色素生成。
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