Njikam Jacqueline, Joshi Apeksha, Njoya Emmanuel M, Upadhyay Kapil, Ngnameko Corinne R, McGaw Lyndy J, Devkar Ranjitsinh V, Njayou Frederic N, Moundipa Paul F
Laboratory of Pharmacology and Toxicology, Department of Biochemistry, Faculty of Science, University of Yaoundé I, PO. Box 812 Yaoundé, Cameroon.
Division of Phytotherapeutics and Metabolic Endocrinology, Department of Zoology, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara, 390002, India.
Curr Drug Res Rev. 2025;17(1):113-125. doi: 10.2174/0125899775282636240307114735.
(HM) and (PA), used traditionally for skin care, have been reported to upregulate the expression of intracellular antioxidant genes, thereby preventing melanoma and protecting fibroblast cell lines from Ultraviolet B (UVB)-induced intracellular oxidative stress.
This investigation aimed to identify major compounds in bioactive fractions using bioassay- guided fractionation.
The anti-inflammatory effect of fractions was determined by measuring their inhibitory activity on 15-lipoxygenase and nitric oxide (NO) in lipopolysaccharide-stimulated RAW 264.7 macrophage cells. Additionally, the anti-aging efficacy of the fractions was determined by assessing the expression of markers for the aging process, i.e., expression of tyrosinase (), tyrosinase-related protein-1 (), procollagen type-1 (), and matrix metalloproteinase- 1 () in UVB-induced photoaging in skin cell-lines. Furthermore, UHPLCMS- based identification of the bioactive compounds from the most prominent fraction was also carried out.
Hexane fraction of HM significantly inhibited (p < 0.05) the 15-lipoxygenase (IC = 46.80 μg/mL) and NO production (IC = 66.55 μg/mL), whereas hexane fraction of PA was effective (p < 0.05) in inhibiting 15-lipoxygenase activity (IC = 27.55 μg/mL). Furthermore, the hexane fraction of HM and methanol fraction of PA were significantly effective (p < 0.05) in reverting the UVB-mediated altered expressions of , and . Furthermore, hexane fraction of HM revealed the presence of harunganin and betulinic acid, whereas vismion D, vismin, kenganthranol B, and bianthrone 1a were identified from the methanol fraction of PA.
Overall, the hexane fraction of HM and methanol fraction of PA displayed effective anti-aging activities, with additional anti-inflammatory effects.
传统上用于皮肤护理的（HM）和（PA）已被报道可上调细胞内抗氧化基因的表达，从而预防黑色素瘤并保护成纤维细胞系免受紫外线B（UVB）诱导的细胞内氧化应激。
本研究旨在通过生物测定导向分级分离法鉴定生物活性组分中的主要化合物。
通过测量各组分对脂多糖刺激的RAW 264.7巨噬细胞中15-脂氧合酶和一氧化氮（NO）的抑制活性来确定其抗炎作用。此外，通过评估衰老过程标志物的表达，即UVB诱导的皮肤细胞系光老化中酪氨酸酶（）、酪氨酸酶相关蛋白-1（）、I型前胶原（）和基质金属蛋白酶-1（）的表达，来确定各组分的抗衰老功效。此外，还基于超高效液相色谱-质谱法对最显著组分中的生物活性化合物进行了鉴定。
HM的己烷组分显著抑制（p < 0.05）15-脂氧合酶（IC = 46.80 μg/mL）和NO生成（IC = 66.55 μg/mL），而PA的己烷组分在抑制15-脂氧合酶活性方面有效（p < 0.05）（IC = 27.55 μg/mL）。此外，HM的己烷组分和PA的甲醇组分在逆转UVB介导的、和表达改变方面显著有效（p < 0.05）。此外，HM的己烷组分中存在哈伦加宁和桦木酸，而从PA的甲醇组分中鉴定出了维斯米翁D、维斯明、肯甘三醇B和双蒽酮1a。
总体而言，HM的己烷组分和PA的甲醇组分显示出有效的抗衰老活性，并具有额外的抗炎作用。
