初乳中针对新生儿感染相关细菌的免疫球蛋白A分析。
Analysis of colostrum IgA against bacteria involved in neonatal infections.
作者信息
Dias Elizabeth Moreira, Rodrigues Denise Bertulucci Rocha, Geraldo-Martins Vinícius Rangel, Nogueira Ruchele Dias
机构信息
Universidade de Uberaba, Uberaba, MG, Brazil.
出版信息
Einstein (Sao Paulo). 2017 Jul-Sep;15(3):256-261. doi: 10.1590/S1679-45082017AO3958.
OBJECTIVE
To describe e compare the specificity of IgA antibodies against bacteria extract of Klebsiella pneumoniae , Staphylococcus aureus , Escherichia coli , and Salmonella enteritidis .
METHODS
Colostrum samples were aseptically collected in the first 12 hours after C-section delivery. The specificity of IgA against bacteria extracts was analyzed by the Western blot.
RESULTS
The findings showed proteins of high molecular weight frequently detectable in the samples. S. aureus was the most frequently found bacterium in the samples (p<0.05). Approximately 93.8, 56.3, 62.5 and 60.4% of samples presented IgA reactive to S. aureus , K. pneumoniae , S. enteritidis, and E. coli, respectively. Roughly 40% of samples showed no IgA reactive to K. pneumoniae, S. enteritidis and E. coli .
CONCLUSION
Clinical evidence of the importance of breastfeeding for the immune protection of neonates was consistent with the observed immunological findings, since most samples showed IgA reactive against the species tested. The application and development of immunotherapies during pregnancy, focused on frequently detected antigens, could be an important tool to enhance the presence of IgA in colostrum.
目的
描述并比较抗肺炎克雷伯菌、金黄色葡萄球菌、大肠杆菌和肠炎沙门氏菌细菌提取物的IgA抗体的特异性。
方法
在剖宫产术后12小时内无菌采集初乳样本。通过蛋白质印迹法分析抗细菌提取物IgA的特异性。
结果
研究结果显示样本中经常可检测到高分子量蛋白质。金黄色葡萄球菌是样本中最常发现的细菌（p<0.05）。分别约93.8%、56.3%、62.5%和60.4%的样本呈现出对抗金黄色葡萄球菌、肺炎克雷伯菌、肠炎沙门氏菌和大肠杆菌的IgA反应性。约40%的样本未显示出对抗肺炎克雷伯菌、肠炎沙门氏菌和大肠杆菌的IgA反应性。
结论
母乳喂养对新生儿免疫保护重要性的临床证据与观察到的免疫学结果一致，因为大多数样本显示出对抗所检测菌种的IgA反应性。孕期针对频繁检测到的抗原进行免疫疗法的应用和开发，可能是增加初乳中IgA含量的重要工具。
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