Study on miRNAs' expression for the invasion of pituitary adenomas.

AIM

To explore the possible invasive effect of four microRNAs in invasive pituitary adenomas.

MATERIAL AND METHODS

Based on our previous studies, several in silico algorithms, and relative literatures, 30 Han Chinese patients with invasive pituitary adenomas and 30 with non-invasive pituitary adenomas were involved in this research. The proteins related to invasion underwent immunohistochemical staining, including basic fibroblast growth factor (FGF2), pituitary tumor transforming gene (PTTG), cyclin B1 (CCNB1), survivin, focal adhesion kinase (FAK), and microvessel density (MVD). To validate the effect of microRNAs, miR-24, miR-93, miR-126, and miR-34a were chosen as possible targets for the aforementioned proteins with four in silico algorithms. All microRNAs tests were performed using quantitative real-time polymerase chain reaction (qPCR).

RESULTS

In our study, FGF2, FAK, PTTG, CCNB1, and MVD were overexpressed in the invasive group compared with the non-invasive group, while an increase in the expression of survivin in the invasive group did not achieve statistical significance. This paper reviewed the literature, and four microRNAs involving invasion were selected for study: miR-24, miR-34a, miR-93, and miR-126. Under-expression of miR-24, miR-34a, and miR-93 was significant in the invasive group, while a decrease of miR-126 expression in the invasive group did not achieve statistical significance.

CONCLUSION

FGF2, PTTG, CCNB1, survivin, FAK, and MVD proteins of pituitary adenoma showed strong expression in invasive tumors. Furthermore, miR-24, miR-93, miR-34a, and miR-126 were under-expressed in invasive Pituitary adenomas compared with non-invasive ones. The results indicated some relationship between the miRNA and protein expression during the pituitary invasion process.