Division of Host Defense, Medical Institute of Bioregulation, Kyushu University, Fukuoka 812-8582, Japan.
Division of Host Defense, Medical Institute of Bioregulation, Kyushu University, Fukuoka 812-8582, Japan.
Cell Rep. 2017 Oct 31;21(5):1191-1202. doi: 10.1016/j.celrep.2017.10.007.
We recently found that a unique subset of innate-like γδ T cells develops from the DN2a stage of the fetal thymus independently of the zinc-finger transcription factor B cell leukemia/lymphoma 11b (Bcl11b). Herein, we characterize these Bcl11b-independent γδ T cells in the periphery as CD5NK1.1 and Granzyme B, and we show that they are capable of producing interferon (IFN)-γ upon T cell receptor stimulation without Ca influx. In wild-type mice, these cells were sparse in lymphoid tissues but abundant in non-lymphoid tissues, such as the liver. Bcl11b-independent CD5NK1.1 γδ T cells appeared and contributed to early protection before Bcl11b-dependent CD5NK1.1 γδ T cells following Listeria monocytogenes infection, resembling their sequential appearance during development in the thymus.
我们最近发现，一类独特的固有样 γδ T 细胞从胎儿胸腺的 DN2a 阶段独立于锌指转录因子 B 细胞白血病/淋巴瘤 11b（Bcl11b）发育而来。在此，我们将外周血中的这些不依赖于 Bcl11b 的 γδ T 细胞鉴定为 CD5NK1.1 和 Granzyme B，并且表明它们能够在 T 细胞受体刺激下产生干扰素（IFN）-γ，而无需钙内流。在野生型小鼠中，这些细胞在淋巴组织中稀少，但在非淋巴组织中丰富，如肝脏。不依赖于 Bcl11b 的 CD5NK1.1 γδ T 细胞在李斯特菌感染后，在依赖于 Bcl11b 的 CD5NK1.1 γδ T 细胞之前出现并有助于早期保护，类似于它们在胸腺发育过程中的顺序出现。
