Bhattacharya S, Glucksberg M R, Bhattacharya J
St. Luke's-Roosevelt Hospital Center, New York, NY 10019.
Circ Res. 1989 Jan;64(1):167-72. doi: 10.1161/01.res.64.1.167.
We have developed a micropuncture technique for the measurement of microvascular pressure in the intact lung of rabbit. We anesthetized 16 rabbits (halothane, 0.8%) and mechanically ventilated them through a tracheotomy. Then, we exposed the right lung by rib resection over the right anterior chest. We measured pulmonary artery, left atrial, and systemic pressures by direct catheterization and cardiac output by the thermodilution technique. For micropuncture, we stabilized the lung on a shelf and stopped ventilation for 3-4 minutes at an airway pressure of 7 cm H2O. We injected pancuronium intravenously to paralyze the diaphragm and intercostal muscles. Of the total pulmonary vascular pressure drop, 52% occurred in the microvascular segment between arterioles and venules of 20 micron diameter, 28% occurred in the arterial segment, and 20% occurred in the venous segment. We conclude that in the intact lung of rabbit, the major pressure drop occurs in the microvascular segment.
我们开发了一种用于测量兔完整肺微血管压力的微穿刺技术。我们将16只兔子麻醉（氟烷，0.8%），并通过气管切开术对其进行机械通气。然后，我们通过切除右前胸的肋骨来暴露右肺。我们通过直接插管测量肺动脉、左心房和体循环压力，并通过热稀释技术测量心输出量。对于微穿刺，我们将肺固定在一个架子上，并在气道压力为7 cm H2O的情况下停止通气3 - 4分钟。我们静脉注射泮库溴铵使膈肌和肋间肌麻痹。在总的肺血管压力降中，52%发生在直径为20微米的小动脉和小静脉之间的微血管段，28%发生在动脉段，20%发生在静脉段。我们得出结论，在兔的完整肺中，主要的压力降发生在微血管段。
