Greenfield Edward A
Cold Spring Harb Protoc. 2017 Nov 1;2017(11):pdb.prot100362. doi: 10.1101/pdb.prot100362.
Immunologically active organs such as the spleen and lymph nodes are rich sources of antibodies; they are also major sites in the body where antibody-producing B cells accumulate. The lymph nodes, in particular, can be targeted when deciding where to inject an animal with antigen. Once an immunized animal has developed a sufficient serum antibody titer, these organs can be harvested for hybridoma fusion and monoclonal antibody production.
免疫活性器官，如脾脏和淋巴结，是抗体的丰富来源；它们也是体内产生抗体的B细胞聚集的主要部位。特别是在决定给动物注射抗原的部位时，可以将淋巴结作为靶点。一旦免疫动物产生了足够的血清抗体滴度，就可以采集这些器官用于杂交瘤融合和单克隆抗体的生产。
