Department of Interventional Therapy, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, 17 Panjiayuan Nanli, Chaoyang District, Beijing, 100021, China.
Surg Endosc. 2022 Jan;36(1):66-74. doi: 10.1007/s00464-020-08238-4. Epub 2021 Jan 4.
To evaluate the efficacy of a silver nanoparticle (AgNP)-coated self-expandable metallic stent (SEMS) for suppressing tissue hyperplasia in a rat esophageal model.
Twenty-four male Sprague-Dawley rats were randomly assigned to four groups. Animals in group A underwent uncoated SEMS placement, whereas animals in groups B, C, and D underwent 6, 12, and 24 mg/mL AgNP-coated SEMS placement, respectively. All animals were euthanized 4 weeks after SEMS placement, and a gross examination and histological analyses were performed.
All rats achieved technical success and survived until the end of the study. The gross examination showed moderate to severe tissue hyperplasia in 5 rats in group A and 2 rats in group B. In contrast, no animals in groups C and D had moderate or severe tissue hyperplasia. The gross examination revealed no complications. The percentage of granulation tissue area, number of epithelial layers, thickness of submucosal fibrosis, percentage of connective tissue area, inflammatory cell infiltration grade, degree of collagen deposition, and degrees of Ki67, TUNEL, and α-SMA-positive deposition were significantly lower in groups C and D than in group A (all p < 0.05). However, only the percentage of granulation tissue area, number of epithelial layers, thickness of submucosal fibrosis, and percentage of connective tissue area were significantly lower in group B than in group A (all p < 0.05). No histological parameters were significantly different between group D and group C (all p > 0.05).
AgNP-coated SEMSs suppressed tissue hyperplasia in a rat esophageal model.
评估载银纳米颗粒（AgNP）涂层自膨式金属支架（SEMS）抑制大鼠食管模型组织增生的疗效。
24 只雄性 Sprague-Dawley 大鼠随机分为 4 组。A 组动物行未涂层 SEMS 放置，B、C 和 D 组动物分别行 6、12 和 24mg/mlAgNP 涂层 SEMS 放置。所有动物于 SEMS 放置后 4 周处死，进行大体检查和组织学分析。
所有大鼠均获得技术成功并存活至研究结束。大体检查显示 A 组 5 只和 B 组 2 只大鼠有中重度组织增生。相比之下，C 组和 D 组无中重度组织增生。大体检查未见并发症。C 组和 D 组的肉芽组织面积百分比、上皮层数、黏膜下层纤维化厚度、结缔组织面积百分比、炎症细胞浸润程度、胶原沉积程度以及 Ki67、TUNEL 和α-SMA 阳性沉积程度均明显低于 A 组（均 p<0.05）。但仅 B 组的肉芽组织面积百分比、上皮层数、黏膜下层纤维化厚度和结缔组织面积百分比明显低于 A 组（均 p<0.05）。D 组与 C 组之间的组织学参数无显著差异（均 p>0.05）。
AgNP 涂层 SEMS 抑制了大鼠食管模型的组织增生。
