Jones B M, Evans P M, Lee D A
Department of Zoology, University College of Wales, Aberystwyth, United Kingdom.
Exp Cell Res. 1989 Jan;180(1):287-96. doi: 10.1016/0014-4827(89)90233-4.
Single-cell suspensions prepared from 9-day-old chick tissues (skeletal muscle, liver, and neural retina) were used to investigate a possible relationship between intrinsic mobilities of different cell types and their positioning behavior in mixed (heterotypic) cellular aggregates. The relative mobilities of the three cell types, determined by comparing their ability to migrate under an agarose layer, was muscle greater than liver greater than neural retina. The gyratory shaker method was employed to produce heterotypic aggregates from mixed suspensions of muscle, liver, and neural retina cells and the tissue-specific positioning of cells after 24 h in culture was determined from histological and autoradiograph sections. The hierarchy for "inside" positioning of segregated cells was muscle greater than liver greater than neural retina cells, correlating with the rate of movement of these cells in the migration assay. The implication of the results is that relative speed of movement may determine the positioning of cells in heterotypic aggregates.
从9日龄鸡的组织（骨骼肌、肝脏和神经视网膜）制备的单细胞悬液，用于研究不同细胞类型的内在迁移能力与其在混合（异型）细胞聚集体中的定位行为之间的可能关系。通过比较三种细胞类型在琼脂糖层下迁移的能力来确定它们的相对迁移率，结果是肌肉细胞大于肝细胞大于神经视网膜细胞。采用回转振荡器法从肌肉、肝脏和神经视网膜细胞的混合悬液中产生异型聚集体，并通过组织学和放射自显影片断确定培养24小时后细胞的组织特异性定位。分离细胞“内部”定位的层次结构是肌肉细胞大于肝细胞大于神经视网膜细胞，这与这些细胞在迁移试验中的移动速率相关。结果表明，相对移动速度可能决定细胞在异型聚集体中的定位。
