异型细胞聚集体中黏附性细胞连接的超微结构研究。
A fine structural study of adhesive cell junctions in heterotypic cell aggregates.
作者信息
Armstrong P B
出版信息
J Cell Biol. 1970 Oct;47(1):197-210. doi: 10.1083/jcb.47.1.197.
Abstract
Mixed suspensions of cells obtained by dissociation of 7 day chicken embryo heart and pigmented retina were allowed to reaggregate in tissue culture. The reaggregates which resulted contained both kinds of cells. Establishment of homogeneous tissues by cell sorting out in these reaggregates was advanced by 20 hr in culture and was complete within 2 days. When sorting out was advanced, heterotypic aggregates were fixed, sectioned, and examined in the electron microscope. Particular attention was paid to the morphology of regions of contact between cells. No qualitative differences were observed in the contact junctions between like cells (heart-heart or pigmented retina-pigment retina junctions) and unlike cells (heart-pigmented retina junctions). Broad areas of undifferentiated cell contact with cell membranes separated by a 100-200 A gap were formed regardless of cell type. Specialized junctions of the fascia and macula adherens type were also present, not only between like cells but also between unlike cells.
摘要
将7日龄鸡胚心脏和色素视网膜解离后获得的细胞混合悬液，在组织培养中使其重新聚集。形成的重新聚集物包含两种细胞。通过细胞分选在这些重新聚集物中建立同质组织在培养中提前了20小时，并在2天内完成。当分选提前时，异型聚集物被固定、切片并在电子显微镜下检查。特别关注细胞间接触区域的形态。在同类细胞（心脏-心脏或色素视网膜-色素视网膜连接）和不同类细胞（心脏-色素视网膜连接）之间的接触连接中未观察到定性差异。无论细胞类型如何，都会形成由100-200埃间隙分隔的细胞膜的未分化细胞广泛接触区域。不仅在同类细胞之间，而且在不同类细胞之间也存在粘着小带和粘着斑类型的特化连接。
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