用于从鼠肠道中富集梭菌的孢子制备方案
Spore Preparation Protocol for Enrichment of Clostridia from Murine Intestine.
作者信息
Velazquez Eric M, Rivera-Chávez Fabian, Bäumler Andreas J
机构信息
Department of Medical Microbiology and Immunology, School of Medicine, University of California at Davis, One Shields Avenue, Davis, CA 95616, USA.
出版信息
Bio Protoc. 2017 May 20;7(10). doi: 10.21769/BioProtoc.2296.
Abstract
In recent years, many spore-forming commensal Clostridia found in the gut have been discovered to promote host physiology, immune development, and protection against infections. We provide a detailed protocol for rapid enrichment of spore-forming bacteria from murine intestine. Briefly, contents from the intestinal cecum are collected aerobically, diluted and finally treated with chloroform to enrich for Clostridia spores.
摘要
近年来，人们发现肠道中许多形成孢子的共生梭菌可促进宿主生理功能、免疫发育并抵御感染。我们提供了一份从鼠肠道中快速富集形成孢子细菌的详细方案。简要来说，需在有氧条件下收集肠道盲肠内容物，进行稀释，最后用氯仿处理以富集梭菌孢子。
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