Department of Chemistry, Purdue University, West Lafayette, IN 47907, USA.
Analyst. 2017 Nov 20;142(23):4373-4387. doi: 10.1039/c7an00985b.
Phosphorylation has an incredible impact on the biological behavior of proteins, altering everything from intrinsic activity to cellular localization and complex formation. It is no surprise then that this post-translational modification has been the subject of intense study and that, with the advent of faster, more accurate instrumentation, the number of large-scale mass spectrometry-based phosphoproteomic studies has swelled over the past decade. Recent developments in sample preparation, phosphorylation enrichment, quantification, and data analysis strategies permit both targeted and ultra-deep phosphoproteome profiling, but challenges remain in pinpointing biologically relevant phosphorylation events. We describe here technological advances that have facilitated phosphoproteomic analysis of cells, tissues, and biofluids and note applications to neuropathologies in which the phosphorylation machinery may be dysregulated, much as it is in cancer.
磷酸化对蛋白质的生物学行为有巨大影响，从固有活性到细胞定位和复合物形成，一切都受到影响。因此，这种翻译后修饰成为了深入研究的主题，并且随着更快、更准确的仪器的出现，基于大规模质谱的磷酸蛋白质组学研究数量在过去十年中激增。在样品制备、磷酸化富集、定量和数据分析策略方面的最新进展允许进行靶向和超深度磷酸蛋白质组学分析，但在确定生物学上相关的磷酸化事件方面仍然存在挑战。我们在这里描述了促进细胞、组织和生物流体的磷酸蛋白质组分析的技术进步，并注意到这些技术在神经病理学中的应用，其中磷酸化机制可能失调，就像在癌症中一样。
