Whitmire W M, Kyle J E, Speer C A
Veterinary Research Laboratory, Montana State University, Bozeman 59717.
Infect Immun. 1989 Jan;57(1):289-90. doi: 10.1128/iai.57.1.289-290.1989.
Autoradiography identified six 125I-labeled proteins, ranging in molecular weight (Mr) from 20,000 to approximately 110,000, on the plasmalemma of Eimeria bovis sporozoites. Immunoblotting with bovine antiserum generated by intravenous inoculations of sporozoites and with immune serum generated by per os inoculations of oocysts revealed that protein 20 (i.e., 20,000 Mr) was an immunodominant antigen on the surface of E. bovis sporozoites.
放射自显影鉴定出牛艾美耳球虫子孢子质膜上有6种125I标记的蛋白质，其分子量（Mr）从20,000到约110,000不等。用静脉接种子孢子产生的牛抗血清以及经口接种卵囊产生的免疫血清进行免疫印迹分析表明，蛋白质20（即20,000 Mr）是牛艾美耳球虫子孢子表面的一种免疫显性抗原。
