Karkhanis Y D, Nollstadt K A, Bhogal B S, Ravino O, Pellegrino R, Crane M S, Murray P K, Turner M J
Department of Biochemical Parasitology, Merck Sharp & Dohme Research Laboratories, Rahway, New Jersey 07065.
Infect Immun. 1991 Mar;59(3):983-9. doi: 10.1128/iai.59.3.983-989.1991.
Chickens were partially protected against coccidiosis induced by Eimeria tenella by using extracts prepared from sonicated sporulated oocysts or from sonicated sporozoites. Following gel filtration of either extract, most of the protective antigens were confined to a single pool of proteins in the molecular mass range between 20 and 30 kDa. Further purification of proteins from the protective pool of sporulated oocyst extract yielded a protective polypeptide with a molecular mass of 26 kDa. An antiserum raised to this pool identified a polypeptide with a molecular mass of 22 kDa in the protective pool from a similarly prepared extract of sporozoites of E. tenella. In subsequent studies, this antiserum was used as an aid in cloning protective polypeptides.
通过使用从超声处理的孢子化卵囊或超声处理的子孢子制备的提取物，鸡对柔嫩艾美耳球虫诱导的球虫病有部分保护作用。对任一提取物进行凝胶过滤后，大多数保护性抗原都集中在分子量范围为20至30 kDa的单一蛋白质池中。从孢子化卵囊提取物的保护性蛋白池中进一步纯化蛋白质，得到了一种分子量为26 kDa的保护性多肽。用针对该蛋白池制备的抗血清在来自类似制备的柔嫩艾美耳球虫子孢子提取物的保护性蛋白池中鉴定出一种分子量为22 kDa的多肽。在随后的研究中，该抗血清被用于辅助克隆保护性多肽。
