Pu Fan, Zhang Wenpeng, Bateman Kevin P, Liu Yong, Helmy Roy, Ouyang Zheng
State Key Laboratory of Precision Measurement Technology & Instruments, Department of Precision Instrument, Tsinghua University, Beijing 100084, PR China.
Department of Chemistry, Purdue University, West Lafayette, IN 47907, USA.
Bioanalysis. 2017 Nov;9(21):1633-1641. doi: 10.4155/bio-2017-0160. Epub 2017 Nov 2.
Preclinical pharmacokinetic studies are an essential part of modern drug development. In this work, we explored a new solution with onsite analysis using a miniature MS system, which can significantly improve the efficiency of the preclinical study. Materials & methods: A miniature mass spectrometer was used with an automatic blood sampler for onsite quantitation of drug compounds in whole blood samples. Slug-flow microextraction was used to replace the in-lab sample preparation.
RESULTS & CONCLUSION: Animal studies were carried out using two drug compounds, using the auto sampler to take blood samples at preprogrammed time points. The miniature MS system was used to obtain drug concentrations, which were subsequently used to calculate the pharmacokinetic parameters.
临床前药代动力学研究是现代药物研发的重要组成部分。在本研究中，我们探索了一种使用微型质谱系统进行现场分析的新方法，该方法可显著提高临床前研究的效率。材料与方法：使用微型质谱仪与自动血液采样器对全血样本中的药物化合物进行现场定量分析。采用段塞流微萃取技术替代实验室样本制备方法。
使用两种药物化合物进行动物研究，通过自动采样器在预设时间点采集血样。利用微型质谱系统获取药物浓度，随后用于计算药代动力学参数。
