Li Linfan, Chen Tsung-Chi, Ren Yue, Hendricks Paul I, Cooks R Graham, Ouyang Zheng
Weldon School of Biomedical Engineering, Purdue University , West Lafayette, IN 47907, United States.
Anal Chem. 2014 Mar 18;86(6):2909-16. doi: 10.1021/ac403766c. Epub 2014 Feb 24.
A benchtop miniature mass spectrometer system, Mini 12, with ambient ionization source and tandem mass spectrometry capabilities has been developed and characterized. This instrument was developed as a self-contained system to produce quantitative results for unprocessed samples of small volumes including nonvolatile analytes. The ion processing system, vacuum system, and control system are detailed. An integrated sample loading system facilitates automated operation. A user interface has been developed to acquire and to interpret analytical results for personnel who have limited mass spectrometry knowledge. Peak widths of Δm/z 0.6 Th (full width at half-maximum) and a mass range of up to m/z 900 are demonstrated with the rectilinear ion trap mass analyzer. Multistage experiments up to MS(5) are accomplished. Consumable cartridges have been designed for use in ambient paper spray ionization, and the recently developed extraction spray ionization method has been employed to improve the quantitative performance. Monitoring of trace-levels of chemicals in therapeutic drugs, as well as in food safety and environmental protection operations is demonstrated. Dual MS/MS scans are implemented to obtain the intensities of the fragment ions from the analyte and its internal standard, and the ratio is used in quantitative analysis of complex samples. Limits of quantitation (LOQ) of 7.5 ng/mL, with relative standard deviations below 10%, have been obtained for selected therapeutic drugs in whole blood throughout their individual therapeutic ranges.
已开发并表征了一种具有常压电离源和串联质谱功能的台式微型质谱仪系统Mini 12。该仪器被开发为一个独立的系统，用于对包括非挥发性分析物在内的小体积未处理样品产生定量结果。详细介绍了离子处理系统、真空系统和控制系统。集成的样品加载系统便于自动化操作。已开发了一个用户界面，供质谱知识有限的人员获取和解释分析结果。使用直线离子阱质量分析仪展示了Δm/z 0.6 Th（半高宽）的峰宽和高达m/z 900的质量范围。完成了高达MS(5)的多级实验。已设计了用于常压纸喷雾电离的消耗性药筒，并采用了最近开发的萃取喷雾电离方法来提高定量性能。展示了在治疗药物、食品安全和环境保护操作中对痕量化学物质的监测。实施了双重MS/MS扫描以获得分析物及其内标物的碎片离子强度，该比率用于复杂样品的定量分析。在全血中选定治疗药物的整个治疗范围内，已获得7.5 ng/mL的定量限（LOQ），相对标准偏差低于10%。
