Zhang Ri-Xin, Zheng Zhi, Li Kai, Wu Xin-Hua, Zhu Ling
Department of Hepatobiliary & Pancreatic Surgery, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Medicine (Baltimore). 2017 Nov;96(44):e8267. doi: 10.1097/MD.0000000000008267.
The purpose of this study was to investigate the association of tumor tissue and plasma miR-146a/b expressions with the clinicopathological properties and overall survival (OS) in surgical patients with intrahepatic cholangiocarcinomas (ICC).Eighty-seven patients with ICC were enrolled. Tumor tissue and plasma sample were collected and miR-146a/b expressions were assessed by quantitative polymerase chain reaction (qPCR). The median follow-up duration was 31 months, and the last follow-up date was January 2017.miR-146a (P < .001) and miR-146b (P = .006) expressions in tumor tissue were positively associated with that in plasma. Tissue miR-146a was negatively correlated with age (P = .036), poor differentiation (P = .020), N stage (P = .020), and TNM stage (P = .007), as well as ECOG performance (P = .008), whereas plasma miR-146a was inversely associated with N stage (P = .003), TNM stage (P = .003), and ECOG performance (P = .011). Moreover, tissue miR-146b was negatively correlated with gender (P = .043) and T stage (P = .047). Kaplan-Meier curves suggested that high expression of tissue miR-146a (P < .001) and plasma miR-146a (P = .029) were correlated with prolonged OS. Nevertheless, no association of miR-146b expression in tumor tissue (P = .187) and plasma (P = .336) with OS was discovered. Univariate analysis indicated that both tissue miR-146a (P < .001) and plasma miR-146a (P = .035) could predict better OS, whereas multivariate analysis revealed that only tissue miR-146a (P = .001) high expression was an independent factor for prolonged OS.Both plasma and tissue miR-146a expression correlated with favorable OS, whereas only tissue miR-146a was an independent prognostic biomarker in surgical patients with ICC.
本研究旨在探讨肝内胆管癌（ICC）手术患者肿瘤组织和血浆中miR-146a/b表达与临床病理特征及总生存期（OS）的相关性。纳入87例ICC患者。收集肿瘤组织和血浆样本，采用定量聚合酶链反应（qPCR）评估miR-146a/b表达。中位随访时间为31个月，最后随访日期为2017年1月。肿瘤组织中miR-146a（P<0.001）和miR-146b（P=0.006）的表达与血浆中的表达呈正相关。组织miR-146a与年龄（P=0.036）、低分化（P=0.020）、N分期（P=0.020）、TNM分期（P=0.007）以及美国东部肿瘤协作组（ECOG）体能状态（P=0.008）呈负相关，而血浆miR-146a与N分期（P=0.003）、TNM分期（P=0.003）和ECOG体能状态（P=0.011）呈负相关。此外，组织miR-146b与性别（P=0.043）和T分期（P=0.047）呈负相关。Kaplan-Meier曲线表明，组织miR-
