Rienhoff H Y
Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.
J Biol Chem. 1989 Jan 5;264(1):419-25.
We have employed a mouse liver-derived cell line (BNL) to identify DNA sequences in the promoter of the serum amyloid A3 (SAA3) gene essential for expression. In both transient DNA transfection assays and BNL cells stably transformed with SAA3 fusion genes, deletion analysis of the SAA3 promoter indicates that sequences between -185 and -138 base pairs 5' to the transcription initiation site are essential for expression of heterologous genes. A 69-base pair sequence spanning this region markedly augmented expression of the bacterial chloramphenicol transacetylase gene regardless of orientation or position. The homology of this sequence with sequences in the promotors of other genes expressed by liver during inflammation suggests a common mechanism of regulation.
我们利用一种源自小鼠肝脏的细胞系（BNL）来鉴定血清淀粉样蛋白A3（SAA3）基因启动子中对于表达至关重要的DNA序列。在瞬时DNA转染试验以及用SAA3融合基因稳定转化的BNL细胞中，对SAA3启动子的缺失分析表明，转录起始位点5'端-185至-138碱基对之间的序列对于异源基因的表达至关重要。跨越该区域的一段69碱基对序列，无论其方向或位置如何，都能显著增强细菌氯霉素转乙酰基酶基因的表达。该序列与炎症期间肝脏表达的其他基因启动子中的序列同源性表明存在一种共同的调控机制。
