Monsanto Argentina S.R.L., Buenos Aires, Argentina.
AgIdea S.A., Pergamino, Buenos Aires, Argentina.
Pest Manag Sci. 2018 Apr;74(4):905-913. doi: 10.1002/ps.4783. Epub 2018 Jan 30.
Maize technologies expressing Bacillus thuringiensis (Bt) insecticidal proteins are widely used in Argentina to control sugarcane borer (Diatraea saccharalis Fabricius). Unexpected D. saccharalis damage was observed to Bt maize events TC1507 (expressing Cry1F) and MON 89034 × MON 88017 (expressing Cry1A.105 and Cry2Ab2) in an isolated area of San Luis Province. Diatraea saccharalis larvae were sampled from MON 89034 × MON 88017 fields in the area to generate a resistant strain (RR), which was subsequently characterized in plant and diet bioassays.
Survivorship of the RR strain was high on TC1507 leaf tissue, intermediate on MON 89034 × MON 88017, and low on MON 810 (expressing Cry1Ab). The RR strain had high resistance to Cry1A.105 (186.74-fold) and no resistance to Cry2Ab2 in diet bioassays. These results indicate resistance to Cry1F and Cry1A.105 (and likely cross-resistance between them) but not to Cry1Ab or Cry2Ab2. Resistance to MON 89034 × MON 88017 was functionally recessive. Reviews of grower records suggest that resistance initially evolved to Cry1F, conferring cross-resistance to Cry1A.105, with low refuge compliance as the primary cause. A mitigation plan was implemented in San Luis that included technology rotation, field monitoring, and grower education on best management practices (BMPs) including refuges.
In the affected area, the resistance to Cry1F and Cry1A.105 is being managed effectively through use of MON 89034 × MON 88017 and MON 810 in combination with BMPs, and no spread of resistance to other regions has been observed. © 2017 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
在阿根廷，广泛使用表达苏云金芽孢杆菌（Bt）杀虫蛋白的玉米技术来控制甘蔗钻心虫（Diatraea saccharalis Fabricius）。在圣路易斯省的一个隔离区，观察到对 Bt 玉米事件 TC1507（表达 Cry1F）和 MON 89034×MON 88017（表达 Cry1A.105 和 Cry2Ab2）的意外的 D. saccharalis 损害。从该地区的 MON 89034×MON 88017 田间采集 Diatraea saccharalis 幼虫，以产生抗性品系（RR），随后在植物和饮食生物测定中对其进行了表征。
RR 品系在 TC1507 叶片组织上的存活率很高，在 MON 89034×MON 88017 上的存活率中等，在 MON 810（表达 Cry1Ab）上的存活率较低。RR 品系对 Cry1A.105 的抗性很高（186.74 倍），而在饮食生物测定中对 Cry2Ab2 没有抗性。这些结果表明对 Cry1F 和 Cry1A.105（可能存在交叉抗性）具有抗性，但对 Cry1Ab 或 Cry2Ab2 没有抗性。对 MON 89034×MON 88017 的抗性是功能隐性的。对种植者记录的审查表明，抗性最初是针对 Cry1F 进化而来的，对 Cry1A.105 具有交叉抗性，低避难所合规性是主要原因。在圣路易斯实施了一项缓解计划，该计划包括技术轮换、田间监测和对最佳管理实践（BMPs）的种植者教育，包括避难所。
在受影响的地区，通过使用 MON 89034×MON 88017 和 MON 810 与 BMPs 相结合来有效管理对 Cry1F 和 Cry1A.105 的抗性，并且没有观察到对其他地区的抗性传播。 © 2017 作者。Pest Management Science 由 John Wiley & Sons Ltd 代表化学工业协会出版。
