Avallone Bice, Cerciello Raimondo, Cretì Patrizia, Pizzoleo Carmela, Scudiero Rosaria, Tizzano Monica, Panzuto Raffaele, Simoniello Palma, Montinari Maria Rosa, Motta Chiara M
Department of Biology, University of Naples Federico II, Naples, 80134, Italy.
Department of Biological and Environmental Sciences and Technologies, University of Salento, Lecce, Italy.
Aquat Toxicol. 2017 Dec;193:201-209. doi: 10.1016/j.aquatox.2017.10.025. Epub 2017 Oct 28.
The effects of an exposure to cadmium chloride 0.47μM for 150days were studied in kidneys of juveniles Sparus aurata by a multidisciplinary approach so to correlate uptake and detoxification potential to changes in brush border and glycocalyx sugar composition. Results demonstrated that cadmium concentration in kidney significantly increased from day 30 reaching a plateau on day 120 while metallothioneins reached a peak on day 90 and by day 120 were already decreasing to control values. Cytological damage was extensive on day 90, clearly detectable at both structural and ultrastructural levels, in tubular cells and brush-border. Staining with a panel of four lectins revealed a significant increase in N-Ac-Gal and a decrease in mannose in the glycocalyx and the tubular basal membranes. From day 120, when cadmium concentration was high and metallothionein concentration decreasing, a clear recovery was observed in tubular cells morphology and sugar composition. Possible significance of these apparently contrasting data are discussed.
采用多学科方法研究了暴露于0.47μM氯化镉150天对幼年金头鲷肾脏的影响，以便将摄取和解毒潜力与刷状缘和糖萼糖组成的变化相关联。结果表明，肾脏中的镉浓度从第30天开始显著增加，在第120天达到平台期，而金属硫蛋白在第90天达到峰值，到第120天已降至对照值。在第90天，细胞学损伤广泛，在肾小管细胞和刷状缘的结构和超微结构水平上均可清晰检测到。用一组四种凝集素染色显示，糖萼和肾小管基底膜中的N-乙酰半乳糖显著增加，甘露糖减少。从第120天开始，当镉浓度较高且金属硫蛋白浓度下降时，观察到肾小管细胞形态和糖组成明显恢复。讨论了这些明显矛盾的数据的可能意义。
