School of Chemical Engineering, Australian Centre for NanoMedicine (ACN), The University of New South Wales, Sydney, NSW 2052, Australia.
Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, QLD 4072, Australia.
J Colloid Interface Sci. 2018 Feb 15;512:404-410. doi: 10.1016/j.jcis.2017.10.069. Epub 2017 Oct 19.
The mounting interest in layered double hydroxide (LDH) nanoparticles as drug carriers and bio-imaging contrast agents makes biosafety evaluation of LDH essential. Considering the important role of blood circulation in bio-distribution of nanoparticles, the present work evaluated the impact of MgAl-LDHs on key components of the circulatory system, including vascular cells (vascular smooth muscle cells (SMCs) and endothelial cells (HUVECs)), red blood cells (RBCs), and complement activation. The results showed that LDH had no effects on SMCs and HUVECs at concentrations up to 500 and 10 µg/mL respectively, in terms of cell proliferation and viability. LDH (10 µg/mL) did not change either the migration distance or the number of migrating SMCs in culture. Moreover, LDH (400 µg/mL) had a negligible effect on RBCs' lysis, and there was no significant increase in levels of complement activation product, C5a, in the presence of LDH (20 or 200 µg/mL). The low toxicity for vascular cells and blood cells combined with low immunogenicity sheds a light on the biosafety of LDH nanoparticles, and encourages further studies into their biomedical applications.
人们对层状双氢氧化物 (LDH) 纳米粒子作为药物载体和生物成像对比剂的兴趣与日俱增，这使得对 LDH 的生物安全性评估变得至关重要。鉴于血液循环在纳米粒子的生物分布中起着重要作用，本研究评估了 MgAl-LDHs 对循环系统关键成分的影响，包括血管细胞（血管平滑肌细胞 (SMCs) 和内皮细胞 (HUVECs)）、红细胞 (RBCs) 和补体激活。结果表明，在高达 500 和 10μg/mL 的浓度下，LDH 对 SMCs 和 HUVECs 的细胞增殖和活力均没有影响。在培养中，LDH（10μg/mL）既没有改变 SMC 的迁移距离，也没有改变迁移 SMC 的数量。此外，LDH（400μg/mL）对 RBC 的溶血作用影响甚微，并且在存在 LDH（20 或 200μg/mL）的情况下，补体激活产物 C5a 的水平也没有显著增加。LDH 纳米粒子对血管细胞和血细胞的低毒性以及低免疫原性表明其具有生物安全性，这鼓励进一步研究其在生物医学中的应用。
