Hayashi H, Ennis P D, Ariga H, Salter R D, Parham P, Kano K, Takiguchi M
Department of Cell Biology, Stanford University, CA 94305.
J Immunol. 1989 Jan 1;142(1):306-11.
Genes encoding the serologically cross-reactive HLA-B51 and HLA-Bw52 molecules were isolated and the exons sequenced. HLA-B51 genes obtained from Caucasian and Oriental individuals were identical. HLA-Bw52 differs from HLA-B51 by four nucleotide substitutions in exon 2 encoding the alpha 1 domain. These comprise one isolated silent substitution in codon 23 and a cluster of three coding substitutions in codons 63 and 67. Amino acid substitutions of N----E at position 63 and F----S at position 67 are the only differences between HLA-B51 and HLA-Bw52 and these residues are postulated to form HLA-B51 specific epitopes. HLA-B51 could have been formed from HLA-Bw52 by the combination of a genetic exchange with HLA-B8 and a point mutation. Similarity of HLA-B51 and HLA-Bw52 with HLA-Bw58 suggest they also share a common ancestor.
分离出编码血清学交叉反应性HLA - B51和HLA - Bw52分子的基因并对其外显子进行测序。从白种人和东方个体获得的HLA - B51基因是相同的。HLA - Bw52与HLA - B51的区别在于，在编码α1结构域的第2外显子中有4个核苷酸替换。这些替换包括密码子23处一个孤立的沉默替换以及密码子63和67处的三个编码替换簇。第63位的N→E和第67位的F→S氨基酸替换是HLA - B51和HLA - Bw52之间仅有的差异，并且推测这些残基形成HLA - B51特异性表位。HLA - B51可能是由HLA - Bw52通过与HLA - B8的基因交换和一个点突变组合而成。HLA - B51和HLA - Bw52与HLA - Bw58的相似性表明它们也有共同的祖先。
