HLA - B39亚型的分子分析

Molecular analysis of HLA-B39 subtypes.

作者信息

Kato N, Karaki S, Kashiwase K, Müller C, Akaza T, Juji T, Kano K, Takiguchi M

机构信息

Department of Tumor Biology and Immunology, University of Tokyo, Japan.

出版信息

Immunogenetics. 1993;37(3):212-6. doi: 10.1007/BF00191887.

DOI:10.1007/BF00191887

PMID:8420828

Abstract

Serological studies have suggested the presence of a new HLA-B39 subtype (B39.2) in the Japanese population. To identify the new HLA-B39 subtype and compare it with an other HLA-B39 subtype (B39.1), the genes encoding HLA-B39.1 (B39013) and B39.2 (B3902) have been cloned from Japanese. We have sequenced these genes and completed the sequence of HLA-B39.1 (B39011) gene from a Caucasian that was partially sequenced. Comparison of the sequence data revealed that B3902 and B39013 differ by three nucleotide substitutions which result in a two amino acids change at residues 63 and 67, while one silent substitution at codon 312 is found between B39011 and B39013. These results suggest that B3902 has evolved from B39013 rather than B39011.

摘要

血清学研究表明，日本人群中存在一种新的HLA - B39亚型（B39.2）。为了鉴定这种新的HLA - B39亚型并将其与另一种HLA - B39亚型（B39.1）进行比较，已从日本人中克隆出编码HLA - B39.1（B39013）和B39.2（B3902）的基因。我们已对这些基因进行了测序，并完成了来自一名白种人的部分测序的HLA - B39.1（B39011）基因的序列。序列数据比较显示，B3902和B39013存在三个核苷酸替换差异，这导致第63和67位氨基酸发生两处变化，而在B39011和B39013之间密码子312处发现一个沉默替换。这些结果表明，B3902是从B39013而非B39011进化而来的。