Murakami Shiho, Miura Yutaka, Hattori Makoto, Matsuda Hiroshi, Malherbe Christiaan J, Muller Christo J F, Joubert Elizabeth, Yoshida Tadashi
Department of Applied Biological Science, Tokyo University of Agriculture and Technology, Tokyo, Japan.
Division of Animal Life Science, Tokyo University of Agriculture and Technology, Tokyo, Japan.
Planta Med. 2018 Mar;84(5):311-319. doi: 10.1055/s-0043-121270. Epub 2017 Nov 2.
, one of the traditional South African medicinal plants, and other species of the same genus offer noteworthy phenolic profiles, in particular high levels of the anti-allergic xanthone mangiferin. Hot water and 40% ethanol-water (v/v) extracts, prepared from , and were tested for immune-regulating activity using murine splenocytes and mesenteric lymph node cells. The 40% ethanol-water extracts of significantly enhanced production of several types of cytokines, including IL-4, IL-17, and IFN-, by antigen-stimulated splenocytes. A concentration-dependent response was observed, noticeably for IFN- production. The activity of the extracts did not correlate with the content of any of the major phenolic compounds, indicative that other extract constituents also play a role in immunomodulation. Additionally, the increased ratio of CD4CD25Foxp3 Treg cells to total CD4 cells indicated induction of Foxp3 cells when mesenteric lymph node cells were cultured in the presence of these two extracts. This study is the first reporting immunostimulatory activity for , which are widely consumed as the herbal tea known as honeybush, underpinning further investigations into the potential use of its extracts as adjuvants for mucosal immunotherapy.
南非传统药用植物之一，以及同一属的其他物种具有值得关注的酚类成分，尤其是含有高水平的抗过敏氧杂蒽酮芒果苷。从[植物名称未给出]制备的热水提取物和40%乙醇 - 水（v/v）提取物，使用小鼠脾细胞和肠系膜淋巴结细胞测试其免疫调节活性。[植物名称未给出]的40%乙醇 - 水提取物显著增强了抗原刺激的脾细胞产生几种类型细胞因子的能力，包括IL - 4、IL - 17和IFN - [此处IFN后具体物质未给出]。观察到浓度依赖性反应，对于IFN - [此处IFN后具体物质未给出]的产生尤为明显。提取物的活性与任何主要酚类化合物的含量均无相关性，这表明其他提取物成分在免疫调节中也发挥作用。此外，当肠系膜淋巴结细胞在这两种提取物存在的情况下培养时，CD4CD25Foxp3调节性T细胞与总CD4细胞的比例增加，表明诱导了Foxp3细胞。这项研究首次报道了[植物名称未给出]的免疫刺激活性，[植物名称未给出]作为名为蜜树茶的草药茶被广泛饮用，这为进一步研究其提取物作为黏膜免疫疗法佐剂的潜在用途奠定了基础。
