a Department of Medical Physiology, Faculty of Medicine , Cairo University , Cairo , Egypt.
b Department of Medical Education , School of Medicine, California University of Science & Medicine , San Bernardino , CA , USA.
Arch Physiol Biochem. 2018 Oct;124(4):292-299. doi: 10.1080/13813455.2017.1396348. Epub 2017 Nov 2.
Parkinson's disease is a progressive neurodegenerative disorder. Aspartame (l-aspartyl-l-phenylalanine methyl ester), a low calorie sweetener used in foods and beverages.
This study investigated the effect of chronic aspartame intake on Parkinsonism induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP).
Forty-eight mice (24 males and 24 females): control, aspartame, MPTP, and aspartame + MPTP groups tested by Y-maze, stepping, forced swimming and olfactory preference tests. Brain tissues examined for dopamine content, tyrosine hydroxylase, inducible nitric oxide synthase (iNOS), glutathione peroxidase, phosphorylated tau and α-synuclein protein. Histopathological evaluation of brain sections at the level of basal ganglia was done.
Decreased dopamine content, tyrosine hydroxylase expression, glutathione peroxidase expression and increased iNOS, tau and α-synuclein expression in groups received aspartame, MPTP or both agents simultaneously in both males and females group.
Increased dopaminergic degeneration and complications with chronic aspartame consumption and more injury in male groups.
帕金森病是一种进行性神经退行性疾病。阿斯巴甜（L-天冬氨酰-L-苯丙氨酸甲酯）是一种用于食品和饮料的低热量甜味剂。
本研究探讨了慢性阿斯巴甜摄入对 1-甲基-4-苯基-1,2,3,6-四氢吡啶（MPTP）诱导的帕金森病的影响。
48 只小鼠（24 只雄性和 24 只雌性）：通过 Y 迷宫、踏步、强迫游泳和嗅觉偏好测试测试对照组、阿斯巴甜组、MPTP 组和阿斯巴甜+MPTP 组。检测脑组织中多巴胺含量、酪氨酸羟化酶、诱导型一氧化氮合酶（iNOS）、谷胱甘肽过氧化物酶、磷酸化tau 和α-突触核蛋白的表达。在基底神经节水平对脑切片进行组织病理学评估。
雄性和雌性小鼠的阿斯巴甜组、MPTP 组或同时接受两种药物组的多巴胺含量、酪氨酸羟化酶表达、谷胱甘肽过氧化物酶表达降低，iNOS、tau 和α-突触核蛋白表达增加。
慢性阿斯巴甜摄入会增加多巴胺能神经退行性变和并发症，并导致雄性小鼠损伤更严重。
