Khorsandi S E, Taanman J W, Heaton N
Institute of Liver Studies, King's College Hospital, Kings College London, London, SE5 9RS, UK.
Department of Clinical Neurosciences, Institute of Neurology, University College London, London, UK.
BMC Res Notes. 2017 Nov 2;10(1):547. doi: 10.1186/s13104-017-2863-7.
Donor liver function in transplantation is defined by mitochondrial function and the ability of mitochondria to recover from the sequence of warm and/or cold ischemia. Mitochondrial resilience maybe related to assembly and- subunit composition of Complex 1. The aim of this study was to determine if Complex 1 subunit composition was different in donor livers of varying quality and whether oxygen exposure had any effect.
Five human livers not suitable for transplant were split. One half placed in cold static storage and the other half exposed to 40% oxygen for 2 h. Protein was extracted for western blot. Membranes were probed with antibodies against β-actin and the following subunits of Complex 1: MTND1, NDUFA10, NDUFB6 and NDUFV2. No difference in steady state Complex 1 subunit composition was demonstrated between donor livers of varying quality, in terms of steatosis or mode of donation. Neither did exposure to oxygen influence Complex 1 subunit composition. This small observational study on subunit levels suggest that Complex 1 is fully assembled as no degradation of subunits associated with the different parts of the enzyme was seen.
移植供肝的肝功能由线粒体功能以及线粒体从热缺血和/或冷缺血序列中恢复的能力来定义。线粒体弹性可能与复合体I的组装和亚基组成有关。本研究的目的是确定不同质量供肝中复合体I的亚基组成是否存在差异，以及氧气暴露是否有任何影响。
将五个不适用于移植的人类肝脏切开。一半置于冷静态保存，另一半暴露于40%氧气中2小时。提取蛋白质用于蛋白质印迹分析。用抗β-肌动蛋白抗体以及复合体I的以下亚基：MTND1、NDUFA10、NDUFB6和NDUFV2对膜进行检测。在不同质量的供肝之间，就脂肪变性或捐赠方式而言，未显示出稳态复合体I亚基组成存在差异。氧气暴露也未影响复合体I的亚基组成。这项关于亚基水平的小型观察性研究表明，由于未观察到与该酶不同部分相关的亚基降解，复合体I已完全组装。
