Schulze Diabetes Institute, Department of Surgery, University of Minnesota, Minneapolis, MN, USA.
Cryobiology. 2012 Jun;64(3):125-43. doi: 10.1016/j.cryobiol.2012.01.007. Epub 2012 Jan 26.
Improved preservation techniques have the potential to improve transplant outcomes by better maintaining donor organ quality and by making more organs available for allotransplantation. Persufflation, (PSF, gaseous oxygen perfusion) is potentially one such technique that has been studied for over a century in a variety of tissues, but has yet to gain wide acceptance for a number of reasons. A principal barrier is the perception that ex vivo PSF will cause in vivo embolization post-transplant. This review summarizes the extensive published work on heart, liver, kidney, small intestine and pancreas PSF, discusses the differences between anterograde and retrograde PSF, and between PSF and other conventional methods of organ preservation (static cold storage, hypothermic machine perfusion). Prospective implications of PSF within the broader field of organ transplantation, and in the specific application with pancreatic islet isolation and transplant are also discussed. Finally, key issues that need to be addressed before PSF becomes a more widely utilized preservation strategy are summarized and discussed.
改进的保存技术有可能通过更好地维持供体器官质量和使更多的器官可用于同种异体移植来改善移植结果。通气（PSF，气态氧灌注）是一种潜在的此类技术，已在各种组织中研究了一个多世纪，但由于多种原因尚未广泛接受。一个主要障碍是人们认为体外 PSF 会在移植后引起体内栓塞。这篇综述总结了广泛发表的关于心脏、肝脏、肾脏、小肠和胰腺 PSF 的工作，讨论了顺行 PSF 和逆行 PSF 之间以及 PSF 和其他传统器官保存方法（静态冷藏、低温机器灌注）之间的差异。还讨论了 PSF 在器官移植更广泛领域内的预期影响，以及在胰腺胰岛分离和移植的具体应用中的影响。最后，总结和讨论了在 PSF 成为更广泛使用的保存策略之前需要解决的关键问题。
