Ozdemir O, Tuzcu M, Sahin N, Orhan C, Tuzcu Z, Sahin K
Department of Biology, Faculty of Science, Firat University, Elazig, Turkey.
Department of Animal Nutrition and Nutritional Disorders, Faculty of Veterinary Medicine, Firat University, Elazig, Turkey.
Cell Mol Biol (Noisy-le-grand). 2017 Oct 31;63(10):93-98. doi: 10.14715/cmb/2017.63.10.15.
In this study, the effect of the supplemental organic chromium (Cr) forms on the expression of ovarian orexin(hypocretin), glucose transporters (GLUTs), heat shock proteins (HSPs) andnuclear factor-kappaB (NF-κB)were investigated in laying hens (HS). Laying hens (n=1800; 16-wk-old; Lohmann LSL-Lite) were allocated to 6 random groups according to a 2 × 3 factorial trial scheme with two different environmental temperatures [Thermoneutral (TN groups; at either 22±2 °C 24 h/d) and heat stress (HS groups; at 34±2 °C for 8 h/d, 08:00 to 17:00 h, followed by 22°C for 16 h for a period of 12 wks)], andhens reared under both environmental conditions were fed either a basal diet or the basal diet supplemented with 1.600 mg of chromium-picolinate (CrPic, 12.43% Cr) and 0.788 mg of chromium-histidinate (CrHis, 25.22% Cr) per kg of diet, delivering 200 µg elemental Cr per kg diet. HS groups showed decreased levels of orexin and GLUTs(GLUT1, GLUT4), and increased NFκB, HSP60, HSP70 and HSP90 levels compared to the TN groups in ovarian tissue of hens (P < 0.0001 for all).However, dietary chromium supplementation (CrPic-CrHis) increasedorexin and GLUTs levels and significantly reduced the NF-κB and HSPs levels making them closer to those of thermoneutral group (P < 0.0001).In conclusion, CrPic and CrHis showed supported the relief and treatment of stress complications.
在本研究中，研究了补充有机铬（Cr）形式对蛋鸡（热应激状态）卵巢中食欲素（下丘脑泌素）、葡萄糖转运蛋白（GLUTs）、热休克蛋白（HSPs）和核因子-κB（NF-κB）表达的影响。将蛋鸡（n = 1800；16周龄；罗曼LSL-Lite）根据2×3析因试验方案分为6个随机组，有两种不同的环境温度[热中性（TN组；每天24小时处于22±2°C）和热应激（HS组；每天8小时处于34±2°C，08:00至17:00，之后16小时处于22°C，持续12周）]，并且在这两种环境条件下饲养的母鸡分别喂食基础日粮或每千克日粮补充1.600毫克吡啶甲酸铬（CrPic，12.43% Cr）和0.788毫克组氨酸铬（CrHis，25.22% Cr）的基础日粮，每千克日粮提供200微克元素铬。与TN组相比，HS组母鸡卵巢组织中食欲素和GLUTs（GLUT1、GLUT4）水平降低，NFκB、HSP60、HSP70和HSP90水平升高（所有P<0.0001）。然而，日粮补充铬（CrPic - CrHis）可提高食欲素和GLUTs水平，并显著降低NF-κB和HSPs水平，使其更接近热中性组（P<0.0001）。总之，CrPic和CrHis显示有助于缓解和治疗应激并发症。
