Department of Animal Nutrition, Faculty of Veterinary Science, Firat University, 23119, Elazig, Turkey.
Division of Biology, Faculty of Science, Firat University, Elazig, Turkey.
Biol Trace Elem Res. 2019 Jun;189(2):529-537. doi: 10.1007/s12011-018-1485-9. Epub 2018 Aug 21.
In the present study, we investigated the effects of chromium-picolinate (CrPic) and chromium-histidinate (CrHis) on nutrient digestibility and nutrient transporters in laying hens exposed to heat stress (HS). Hens (n = 1800; 16 weeks old) were kept in cages in temperature-controlled rooms at either 22 ± 2 °C for 24 h/day (thermoneutral (TN)) or 34 ± 2 °C for 8 h/day, from 08:00 to 17:00, followed by 22 °C for 16 h (HS) for 12 weeks. Hens reared under both environmental conditions were fed one of three diets: a basal diet and the basal diet supplemented with either 1.600 mg of CrPic (12.43% Cr) or 0.788 mg of CrHis (25.22% Cr) per kg of diet, delivering 200 μg elemental Cr per kg of diet. HS impaired the nutrient digestibility and nutrient transports in laying hens (P < 0.001). However, both Cr sources increased digestibility of dry matter (DM; P < 0.001), organic matter (OM; P < 0.05), crude protein (CP; P < 0.001), and crude fat (CF; P < 0.001). Both Cr sources partially alleviated detrimental effects of HS on fatty acid-binding and transport protein1 (FABP1, FATP1), glucose (SGLT1, GLUT1, GLUT10), protein (PepT1, PepT2), and amino acid transporters (ASCT1, bAT1, CAT1, EAAT1, LAT1) of the ileum (P < 0.0001). The efficacy of Cr as CrHis was more notable than Cr as CrPic, which could be attributed to higher bioavailability. Finally, the detrimental effects of HS on nutrient digestibility and nutrient transporters were alleviated by CrPic and CrHis. These findings may justify the use of CrPic and CrHis in poultry.
在本研究中，我们研究了吡啶羧酸铬（CrPic）和组氨酸铬（CrHis）对暴露于热应激（HS）的产蛋鸡的营养消化率和营养转运体的影响。将 1800 只（16 周龄）母鸡饲养在温度控制的笼中，每天 24 小时保持在 22±2°C（常温（TN））或 34±2°C 8 小时，从 08：00 到 17：00，然后在 12 周内保持 16 小时 22°C（HS）。在两种环境条件下饲养的母鸡分别喂食三种日粮之一：基础日粮和基础日粮分别补充 1.600mg CrPic（12.43% Cr）或 0.788mg CrHis（25.22% Cr）/kg 日粮，每公斤日粮提供 200μg 元素 Cr。HS 降低了产蛋鸡的营养消化率和营养转运体（P<0.001）。然而，两种铬源都提高了干物质（DM；P<0.001）、有机物（OM；P<0.05）、粗蛋白（CP；P<0.001）和粗脂肪（CF；P<0.001）的消化率。两种铬源部分缓解了 HS 对脂肪酸结合蛋白和转运蛋白 1（FABP1、FATP1）、葡萄糖（SGLT1、GLUT1、GLUT10）、蛋白质（PepT1、PepT2）和氨基酸转运体（ASCT1、bAT1、CAT1、EAAT1、LAT1）的有害影响。回肠（P<0.0001）。Cr 作为 CrHis 的功效比 Cr 作为 CrPic 更显著，这可能归因于更高的生物利用度。最后，CrPic 和 CrHis 缓解了 HS 对营养消化率和营养转运体的有害影响。这些发现可能证明 CrPic 和 CrHis 在禽类中的使用是合理的。
