利用线粒体细胞色素c氧化酶I基因对暗黑棉蝽（Oxycarenus hyalinipennis）进行遗传条形码分析和系统发育分析。
Genetic barcoding and phylogenetic analysis of dusky cotton bug (Oxycarenus hyalinipennis) using mitochondrial cytochrome c oxidase I gene.
作者信息
Khan A Z, Amad I, Shaheen S, Hussain K, Hafeez F, Farooq M, Noor Ul Ayan H
机构信息
Plant Research Group, Department of Bioinformatics and Biotechnology, Government College University Faisalabad, Pakistan.
Entomology Research Institute, Ayub Agricultural Research Institute, Faisalabad, Pakistan.
出版信息
Cell Mol Biol (Noisy-le-grand). 2017 Oct 31;63(10):59-63. doi: 10.14715/cmb/2017.63.10.9.
Cotton dusky bug (Oxycarenus spp.) mostly attack on cash crops such as Gossypium, Cola and Hibiscus which affect the national economy therefore sustainable pest management is needed. Cytochrome c oxidase I (COI) gene is utilized as marker gene for DNA barcoding, genetic and ecological study of insects. In present study insect (cotton dusky bug) samples were collected from cotton fields in Faisalabad. COI gene was amplified from genomic DNA of bug and cloned into pTZ57R/T vector (Fermentas). The clone was sent to Macrogen (South Korea) for Sanger sequencing. The phylogenetic analysis and pairwise multiple sequence alignment showed that our cotton dusky bug grouped with two species of Oxycarenus genus and highest sequence identity was 91.1% with Oxycarenus hylinipennis. This is the first report of genetic barcode of Oxycarenus hylinipennis from cotton from Pakistan.
棉暗蝽（Oxycarenus属）主要侵害棉花、可乐果和木槿等经济作物，影响国民经济，因此需要可持续的害虫管理。细胞色素c氧化酶I（COI）基因被用作昆虫DNA条形码、遗传和生态研究的标记基因。在本研究中，从费萨拉巴德的棉田采集了昆虫（棉暗蝽）样本。从蝽的基因组DNA中扩增出COI基因，并克隆到pTZ57R/T载体（Fermentas）中。该克隆被送往韩国Macrogen进行桑格测序。系统发育分析和两两多序列比对表明，我们的棉暗蝽与Oxycarenus属的两个物种聚类，与林栖棉暗蝽的最高序列同一性为91.1%。这是来自巴基斯坦棉花的林栖棉暗蝽遗传条形码的首次报道。
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