Division of Reproductive Sciences, Department of Obstetrics and Gynecology, University of Colorado School of Medicine, 12700 East 19th Ave, Aurora, CO, 80045, USA.
Division of Reproductive Sciences, Department of Obstetrics and Gynecology, University of Colorado School of Medicine, 12700 East 19th Ave, Aurora, CO, 80045, USA; Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, University of Colorado School of Medicine, 12700 East 19th Ave, Aurora, CO, 80045, USA.
Mol Cell Endocrinol. 2018 Jul 15;470:199-207. doi: 10.1016/j.mce.2017.10.016. Epub 2017 Oct 31.
We evaluated the impact of high-fat diet (HFD) on ovarian gene expression. Female 5-week-old C57BL/6J mice were fed a 60% HFD or standard chow for 10 weeks. HFD-fed mice were then separated into obese (HF-Ob) and lean (HF-Ln) based on body weight. HFD exposure led to impairment of the estrous cycle, changes in hormones affecting reproduction, and decreased primordial follicles regardless of the development of obesity. RNA-sequencing of whole ovaries identified multiple genes with altered expression after HFD, with 25 genes displaying decreased expression in both HF-Ln and HF-Ob mice compared to the chow-fed controls (q < 0.05). Several of these 25 genes are involved in normal ovarian functions, including ovulation (Edn2, Tnfaip6, Errfi1, Prkg2, and Nfil3), luteinization (Edn2), and luteolysis (Nr4a1). Taken together, elevated dietary fat intake, regardless of obesity, is associated with impaired estrous cycle, depletion of the ovarian reserve, and altered expression of genes critical to normal ovulatory function.
我们评估了高脂肪饮食（HFD）对卵巢基因表达的影响。将 5 周龄的雌性 C57BL/6J 小鼠用 60%的 HFD 或标准食物喂养 10 周。然后，根据体重将 HFD 喂养的小鼠分为肥胖（HF-Ob）和瘦（HF-Ln）两组。HFD 暴露导致发情周期受损、影响生殖的激素变化以及原始卵泡减少，而与肥胖的发展无关。对整个卵巢的 RNA 测序确定了 HFD 后表达发生改变的多个基因，与喂食标准食物的对照组相比，25 个基因在 HF-Ln 和 HF-Ob 小鼠中均表达下调（q < 0.05）。这些 25 个基因中的几个参与正常卵巢功能，包括排卵（Edn2、Tnfaip6、Errfi1、Prkg2 和 Nfil3）、黄体化（Edn2）和黄体溶解（Nr4a1）。总之，无论肥胖与否，饮食中脂肪摄入的增加都与发情周期受损、卵巢储备减少以及对正常排卵功能至关重要的基因表达改变有关。
