Ding Z Z, Ma J, He W, Ge Z L, Lu Q, Kaplan D L
National Engineering Laboratory for Modern Silk and Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University, Suzhou 215123, People's Republic of China.
Department of Stomatology, The First Affiliated Hospital of Soochow University, Suzhou 215006, People's Republic of China.
J Mater Chem B. 2017 Jun 28;5(24):4789-4796. doi: 10.1039/C7TB00486A. Epub 2017 May 16.
Extracellular matrix (ECM) is a system used to model the design of biomaterial matrices for tissue regeneration. Various biomaterial systems have been developed to mimic the composition or microstructure of the ECM. However, emulating multiple facets of the ECM in these systems remains a challenge. Here, a new strategy is reported which addresses this need by using silk fibroin and chitosan (CS) nanocomposite materials. Silk fibroin was first assembled into ECM-mimetic nanofibers in water and then blended with CS to introduce the nanostructural cues. Then the ratios of silk fibroin and CS were optimized to imitate the protein and glycosaminoglycan compositions. These biomaterial scaffolds had suitable compositions, hierarchical nano-to-micro structures, and appropriate mechanical properties to promote cell proliferation , and vascularization and tissue regeneration . Compared to previous silk-based scaffolds, these scaffolds achieved improvements in biocompatibility, suggesting promising applications in the future in tissue regeneration.
细胞外基质（ECM）是一种用于模拟组织再生生物材料基质设计的系统。已经开发了各种生物材料系统来模仿ECM的组成或微观结构。然而，在这些系统中模拟ECM的多个方面仍然是一个挑战。在此，报道了一种新策略，该策略通过使用丝素蛋白和壳聚糖（CS）纳米复合材料来满足这一需求。丝素蛋白首先在水中组装成模仿ECM的纳米纤维，然后与CS混合以引入纳米结构线索。然后优化丝素蛋白和CS的比例以模仿蛋白质和糖胺聚糖的组成。这些生物材料支架具有合适的组成、分级的纳米到微米结构以及适当的机械性能，以促进细胞增殖、血管生成和组织再生。与以前的丝基支架相比，这些支架在生物相容性方面有了改进，表明在未来组织再生中具有广阔的应用前景。
