Yu Li-Min, Liu Tao, Ma Yu-Long, Zhang Feng, Huang Yong-Can, Fan Zhi-Hai
Shenzhen Key Laboratory of Spine Surgery, Department of Spine Surgery, Peking University Shenzhen Hospital, Shenzhen, China.
Department of Textile Engineering, College of Textile and Clothing Engineering, Soochow University, Suzhou, China.
Front Bioeng Biotechnol. 2020 Dec 11;8:578988. doi: 10.3389/fbioe.2020.578988. eCollection 2020.
Interest is rapidly growing in the design and preparation of bioactive scaffolds, mimicking the biochemical composition and physical microstructure for tissue repair. In this study, a biomimetic biomaterial with nanofibrous architecture composed of silk fibroin and hyaluronic acid (HA) was prepared. Silk fibroin nanofiber was firstly assembled in water and then used as the nanostructural cue; after blending with hyaluronan (silk:HA = 10:1) and the process of freeze-drying, the resulting composite scaffolds exhibited a desirable 3D porous structure and specific nanofiber features. These scaffolds were very porous with the porosity up to 99%. The mean compressive modulus of silk-HA scaffolds with HA MW of 0.6, 1.6, and 2.6 × 10 Da was about 28.3, 30.2, and 29.8 kPa, respectively, all these values were much higher than that of pure silk scaffold (27.5 kPa). This scaffold showed good biocompatibility with bone marrow mesenchymal stem cells, and it enhanced the cellular proliferation significantly when compared with the plain silk fibroin. Collectively, the silk-hyaluronan composite scaffold with a nanofibrous structure and good biocompatibility was successfully prepared, which deserved further exploration as a biomimetic platform for mesenchymal stem cell-based therapy for tissue repair.
目前，人们对生物活性支架的设计和制备兴趣迅速增长，这类支架可模拟组织修复所需的生化组成和物理微观结构。在本研究中，制备了一种由丝素蛋白和透明质酸（HA）组成的具有纳米纤维结构的仿生生物材料。首先在水中组装丝素蛋白纳米纤维，并将其用作纳米结构线索；与透明质酸混合（丝素蛋白：HA = 10:1）并经过冷冻干燥过程后，所得复合支架呈现出理想的三维多孔结构和特定的纳米纤维特征。这些支架孔隙率很高，可达99%。HA分子量分别为0.6、1.6和2.6×10 Da的丝素蛋白-HA支架的平均压缩模量分别约为28.3、30.2和29.8 kPa，所有这些值均远高于纯丝素蛋白支架（27.5 kPa）。该支架与骨髓间充质干细胞具有良好的生物相容性，与普通丝素蛋白相比，能显著促进细胞增殖。总体而言，成功制备了具有纳米纤维结构和良好生物相容性的丝素蛋白-透明质酸复合支架，作为基于间充质干细胞的组织修复治疗的仿生平台，值得进一步探索。
