Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen University, Shenzhen 518060, China.
Analyst. 2017 Nov 20;142(23):4503-4510. doi: 10.1039/c7an01245d.
Optical Coherence Tomography (OCT) is a valuable technology that has been used to obtain microstructure images of tissue, and has several advantages, though its applications are limited in high-scattering tissues. Therefore, semiconducting polymer nanoparticles (SPNs) that possess strong absorption characteristics are applied to decrease light scattering in tissues and used as exogenous contrast agents for enhancing the contrast of OCT imaging detection. In this paper, we prepared two kinds of SPNs, termed PIDT-TBZ SPNs and PBDT-TBZ SPNs, as the contrast agents for OCT detection to enhance the signal. Firstly, we proved that they were good contrast agents for OCT imaging in agar-TiO. After that, the contrast effects of these two SPNs were quantitatively analyzed, and then cerebral blood vessels were monitored by a home-made SD-OCT system. Finally, we created OCT images in vitro and in vivo with these two probes and performed quantitative analysis using the images. The results indicated that these SPNs created a clear contrast enhancement of small vessels in the OCT imaging process, which provides a basis for the application of SPNs as contrast agents for bioimaging studies.
光学相干断层扫描（OCT）是一种有价值的技术，用于获取组织的微观结构图像，具有多个优点，但其应用在高散射组织中受到限制。因此，具有强吸收特性的半导体聚合物纳米粒子（SPN）被应用于减少组织中的光散射，并用作外源性对比剂来增强 OCT 成像检测的对比度。在本文中，我们制备了两种 SPN，分别称为 PIDT-TBZ SPN 和 PBDT-TBZ SPN，作为 OCT 检测的对比剂来增强信号。首先，我们证明了它们在琼脂-TiO 中是良好的 OCT 成像对比剂。之后，对这两种 SPN 的对比效果进行了定量分析，然后使用自制的 SD-OCT 系统监测脑血管。最后，我们使用这两种探针在体外和体内创建了 OCT 图像，并对图像进行了定量分析。结果表明，这些 SPN 在 OCT 成像过程中对小血管产生了清晰的对比度增强，这为 SPN 作为生物成像研究的对比剂的应用提供了依据。
